Immune responses in beta-thalassaemia: heme oxygenase 1 reduces cytokine production and bactericidal activity of human leucocytes.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
24 06 2020
Historique:
received: 18 12 2019
accepted: 08 06 2020
entrez: 26 6 2020
pubmed: 26 6 2020
medline: 22 12 2020
Statut: epublish

Résumé

Patients with beta-thalassaemia increase the risk of bacterial infections, particularly Burkholderia pseudomallei (Bp), the causative agent of melioidosis in Thailand. Impaired immune cell functions may be the cause of this susceptibility, but detailed mechanisms have not been defined. In this study, we observed impaired production of IFN-gamma and IL-10 by whole blood from beta-thalassaemia patients upon stimulation with a range of bacteria-derived stimuli. In contrast, IFN-gamma response via TCR and plasma IgG specific for Bp were still intact. Importantly, mRNA expression of heme oxygenase 1 (HO-1), a potential modulator of immune function, was increased in whole blood from beta-thalassaemia patients, either with or without stimulation with Bp in vitro. Induction of HO-1 by hemin or CoPP in vitro reduced production of IFN-gamma and IL-10 from healthy human PBMCs and decreased bacterial clearance activity of whole blood from healthy controls and beta-thalassaemia, while inhibition of HO-1 by SnPP enhanced both functions in healthy controls. These results were confirmed to some extent in purified human monocytes of healthy controls. Our results suggest a mechanism that excess hemin of beta-thalassaemia patients is a significant cause of immune suppression via HO-1 induction and may underlie the susceptibility of these individuals to severe bacterial infection.

Identifiants

pubmed: 32581238
doi: 10.1038/s41598-020-67346-2
pii: 10.1038/s41598-020-67346-2
pmc: PMC7314746
doi:

Substances chimiques

IFNG protein, human 0
IL10 protein, human 0
RNA, Messenger 0
Interleukin-10 130068-27-8
Interferon-gamma 82115-62-6
HMOX1 protein, human EC 1.14.14.18
Heme Oxygenase-1 EC 1.14.14.18

Types de publication

Journal Article Observational Study Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

10297

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Auteurs

Arnone Nithichanon (A)

The Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.

Inthira Tussakhon (I)

The Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.

Waraporn Samer (W)

The Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.

Chidchamai Kewcharoenwong (C)

The Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand.

Manabu Ato (M)

Department of Immunology, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan.

Gregory J Bancroft (GJ)

Department of Infection Biology, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UK.

Ganjana Lertmemongkolchai (G)

The Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand. ganja_le@kku.ac.th.

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