Multicentre comparison of quantitative PCR-based assays to detect SARS-CoV-2, Germany, March 2020.


Journal

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
ISSN: 1560-7917
Titre abrégé: Euro Surveill
Pays: Sweden
ID NLM: 100887452

Informations de publication

Date de publication:
06 2020
Historique:
entrez: 26 6 2020
pubmed: 26 6 2020
medline: 3 7 2020
Statut: ppublish

Résumé

Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven diagnostic laboratories in Germany in March 2020. While most assays performed well, we identified detection problems in a commonly used assay that may have resulted in false-negative test results during the first weeks of the pandemic.

Identifiants

pubmed: 32583765
doi: 10.2807/1560-7917.ES.2020.25.24.2001057
pmc: PMC7315722
doi:

Types de publication

Comparative Study Journal Article Multicenter Study

Langues

eng

Sous-ensembles de citation

IM

Références

J Clin Microbiol. 2020 May 26;58(6):
pubmed: 32269100
J Pediatric Infect Dis Soc. 2020 May 22;:
pubmed: 32441753
Euro Surveill. 2020 Mar;25(9):
pubmed: 32156330
Nature. 2020 Mar;579(7798):265-269
pubmed: 32015508
J Clin Virol. 2020 Jul;128:104412
pubmed: 32416600
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387

Auteurs

Maximilian Muenchhoff (M)

Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany.
German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.

Helga Mairhofer (H)

Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany.
German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.

Hans Nitschko (H)

Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany.
German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.

Natascha Grzimek-Koschewa (N)

Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany.
German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.

Dieter Hoffmann (D)

German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
Institute of Virology, School of Medicine, Technical University Munich/Helmholtz Zentrum München, Munich, Germany.

Annemarie Berger (A)

German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
Institute of Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany.

Holger Rabenau (H)

German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
Institute of Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany.

Marek Widera (M)

German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
Institute of Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany.

Nikolaus Ackermann (N)

Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.

Regina Konrad (R)

Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.

Sabine Zange (S)

German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
Bundeswehr Institute of Microbiology, Munich, Germany.

Alexander Graf (A)

Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig Maximilian University, Munich, Germany.

Stefan Krebs (S)

Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig Maximilian University, Munich, Germany.

Helmut Blum (H)

Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig Maximilian University, Munich, Germany.

Andreas Sing (A)

Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.

Bernhard Liebl (B)

Bavarian Health and Food Safety Authority, Oberschleißheim, Germany.

Roman Wölfel (R)

German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
Bundeswehr Institute of Microbiology, Munich, Germany.

Sandra Ciesek (S)

German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
Institute of Medical Virology, University Hospital, Goethe University Frankfurt am Main, Frankfurt, Germany.

Christian Drosten (C)

German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
Institute of Virology, Charité University Medicine, Berlin, Germany.

Ulrike Protzer (U)

German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.
Institute of Virology, School of Medicine, Technical University Munich/Helmholtz Zentrum München, Munich, Germany.

Stephan Boehm (S)

Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany.
German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.

Oliver T Keppler (OT)

Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Ludwig Maximilian University, Munich, Germany.
German Center for Infection Research, Partner Site Munich and Associated Partner Site Charité, Berlin and Associated Partner Site Frankfurt, Germany.

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