LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair.
A549 Cells
Antigens, Nuclear
/ genetics
DNA Breaks, Double-Stranded
/ radiation effects
DNA End-Joining Repair
/ genetics
DNA Repair Enzymes
/ genetics
DNA-Binding Proteins
/ deficiency
HEK293 Cells
HeLa Cells
Histones
/ metabolism
Humans
Ku Autoantigen
/ genetics
RNA, Long Noncoding
/ genetics
Signal Transduction
Journal
Cell death and differentiation
ISSN: 1476-5403
Titre abrégé: Cell Death Differ
Pays: England
ID NLM: 9437445
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
15
10
2019
accepted:
12
06
2020
revised:
10
06
2020
pubmed:
27
6
2020
medline:
15
12
2021
entrez:
27
6
2020
Statut:
ppublish
Résumé
Despite recent advances in our understanding of the function of long noncoding RNAs (lncRNAs), their roles and functions in DNA repair pathways remain poorly understood. By screening a panel of uncharacterized lncRNAs to identify those whose transcription is induced by double-strand breaks (DSBs), we identified a novel lncRNA referred to as LRIK that interacts with Ku, which enhances the ability of the Ku heterodimer to detect the presence of DSBs. Here, we show that depletion of LRIK generates significantly enhanced sensitivity to DSB-inducing agents and reduced DSB repair efficiency. In response to DSBs, LRIK enhances the recruitment of repair factors at DSB sites and facilitates γH2AX signaling. Our results demonstrate that LRIK is necessary for efficient repairing DSBs via nonhomologous end-joining pathway.
Identifiants
pubmed: 32587379
doi: 10.1038/s41418-020-0581-5
pii: 10.1038/s41418-020-0581-5
pmc: PMC7852670
doi:
Substances chimiques
Antigens, Nuclear
0
DNA-Binding Proteins
0
H2AX protein, human
0
Histones
0
NHEJ1 protein, human
0
RNA, Long Noncoding
0
XRCC5 protein, human
EC 3.6.4.12
Xrcc6 protein, human
EC 3.6.4.12
Ku Autoantigen
EC 4.2.99.-
DNA Repair Enzymes
EC 6.5.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3337-3353Références
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