Decoration of electrical conductive polyurethane-polyaniline/polyvinyl alcohol matrixes with mussel-inspired polydopamine for bone tissue engineering.
Aniline Compounds
/ pharmacology
Animals
Bivalvia
/ drug effects
Bone Development
/ drug effects
Bone and Bones
/ drug effects
Cell Survival
/ drug effects
Electric Conductivity
Humans
Indoles
/ pharmacology
Mesenchymal Stem Cells
/ drug effects
Polymers
/ pharmacology
Polyurethanes
/ pharmacology
Polyvinyl Alcohol
/ pharmacology
Spectroscopy, Fourier Transform Infrared
Tissue Engineering
/ methods
bone
co-electrospinning
electrical stimulation
scaffold
tissue engineering
Journal
Biotechnology progress
ISSN: 1520-6033
Titre abrégé: Biotechnol Prog
Pays: United States
ID NLM: 8506292
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
06
04
2020
revised:
20
06
2020
accepted:
23
06
2020
pubmed:
28
6
2020
medline:
2
9
2021
entrez:
28
6
2020
Statut:
ppublish
Résumé
Electrospinning is a versatile technology for the fabrication of nanofibrous matrixes to regenerate defects. This study aims to develop a functionalized and electroconductive polymeric matrix to improve rat bone marrow mesenchymal stem cell adhesion, proliferation, and differentiation. Herein, the influence of the chemical composition of the substrate on homogeneous modification of the surface with mussel-inspired polydopamine (PDA) is focused. Accordingly, the deposition of PDA on the surface was proved by Fourier transform infrared spectroscopy. Morphologies of the scaffolds demonstrated homogeneous decoration of the polyvinyl alcohol (PVA)/polyurethane (PU)-polyaniline (PANI) matrixes with PDA, while a lower density of mussel-inspired polymer was observed in bare PU-PANI constructs. Although uniform and dense precipitation of PDA reduced conductivity of scaffolds 1.2 times compared with the samples with a low density of the PDA, 1.1 and 1.2 times enhancement in tensile strength and Young's modulus, respectively, were the strength of the applied process, especially in bone tissue engineering area. Contact angle measurements demonstrated about two times reduction in measured values, which shows improvement in hydrophilicity of PDA-modified PVA/PU-PANI fibers compared with PDA-coated PU-PANI ones. Swelling ratio and mass loss ratio calculations revealed enhancement in measured values as a function of homogeneous and dense coating, which arise from hydrophilicity of the polymeric substrate. The bioactivity test indicated that a dense layer of PDA strongly supports formations of hydroxyapatite-like crystals. Moreover, homogeneous decoration of conductive matrixes with PDA showed suitable cell viability, adhesion, and spreading while cell-scaffolds interactions improved under electrical stimulation. Higher expression of alkaline phosphatase and secretion of Collagen I under the electrical field proved the applicability of modified electroconductive scaffolds for further preclinical and clinical studies to introduce as a reconstructive bone substitute.
Substances chimiques
Aniline Compounds
0
Indoles
0
Polymers
0
Polyurethanes
0
polyaniline
0
polydopamine
0
Polyvinyl Alcohol
9002-89-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3043Subventions
Organisme : Key Disciplines Group Construction Project of Pudong Health Bureau of Shanghai
ID : PWZxq2017-11
Pays : International
Organisme : National Natural Science Foundation of China
ID : 81971753
Pays : International
Organisme : Outstanding Clinical Discipline Project of Shanghai Pudong: PWYgy2018-09
Pays : International
Organisme : Program for Medical Key Department of Shanghai
ID : ZK2019C01
Pays : International
Organisme : Program for Outstanding Leader of Shang
ID : 046
Pays : International
Informations de copyright
© 2020 American Institute of Chemical Engineers.
Références
Vo TN, Kasper FK, Mikos AG. Strategies for controlled delivery of growth factors and cells for bone regeneration. Adv Drug Deliv Rev. 2012;64(12):1292-1309. https://doi.org/10.1016/j.addr.2012.01.016.
Tee R, Lokmic Z, Morrison WA, Dilley RJ. Strategies in cardiac tissue engineering. ANZ J Surg. 2010;80(10):683-693. https://doi.org/10.1111/j.1445-2197.2010.05435.x.
Huang BJ, Hu JC, Athanasiou KA. Cell-based tissue engineering strategies used in the clinical repair of articular cartilage. Biomaterials. 2016;98:1-22. https://doi.org/10.1016/j.biomaterials.2016.04.018.
Bártolo PJ, Domingos M, Patrício T, Cometa S, Mironov V. Biofabrication strategies for tissue engineering. Advances on Modeling in Tissue Engineering. Dordrecht: Springer; 2011:137-176. https://doi.org/10.1007/978-94-007-1254-6_8.
Brunelle AR, Horner CB, Low K, Ico G, Nam J. Electrospun thermosensitive hydrogel scaffold for enhanced chondrogenesis of human mesenchymal stem cells. Acta Biomater. 2018;66:166-176. https://doi.org/10.1016/j.actbio.2017.11.020.
Prabu GTV, Dhurai B. A novel profiled multi-pin electrospinning system for nanofiber production and encapsulation of nanoparticles into nanofibers. Sci Rep. 2020;10(1):4302. https://doi.org/10.1038/s41598-020-60752-6.
Chao CY, Mani MP, Kumar S, Id J. Engineering electrospun multicomponent polyurethane scaffolding platform comprising grapeseed oil and honey/propolis for bone tissue regeneration. 2018:1-17.
Shrestha BK, Shrestha S, Tiwari AP, et al. Bio-inspired hybrid scaffold of zinc oxide-functionalized multi-wall carbon nanotubes reinforced polyurethane nanofibers for bone tissue engineering. Mater des. 2017;133:69-81. https://doi.org/10.1016/j.matdes.2017.07.049.
Kim YS, Cho K, Lee HJ, et al. Highly conductive and hydrated PEG-based hydrogels for the potential application of a tissue engineering scaffold. React Funct Polym. 2016;109:15-22. https://doi.org/10.1016/j.reactfunctpolym.2016.09.003.
Guo B, Glavas L, Albertsson A-C. Biodegradable and electrically conducting polymers for biomedical applications. Prog Polym Sci. 2013;38(9):1263-1286. https://doi.org/10.1016/j.progpolymsci.2013.06.003.
Qazi TH, Rai R, Boccaccini AR. Tissue engineering of electrically responsive tissues using polyaniline based polymers: a review. Biomaterials. 2014;35(33):9068-9086. https://doi.org/10.1016/j.biomaterials.2014.07.020.
Wang L, Wu Y, Hu T, Guo B, Ma PX. Electrospun conductive nanofibrous scaffolds for engineering cardiac tissue and 3D bioactuators. Acta Biomater. 2017;59:68-81. https://doi.org/10.1016/j.actbio.2017.06.036.
Grant C, Twigg P, Egan A, et al. Poly(vinyl alcohol) hydrogel as a biocompatible viscoelastic mimetic for articular cartilage. Biotechnol Prog. 2008;22(5):1400-1406. https://doi.org/10.1021/bp060181u.
Shuai C, Mao Z, Lu H, Nie Y, Hu H, Peng S. Fabrication of porous polyvinyl alcohol scaffold for bone tissue engineering via selective laser sintering. Biofabrication. 2013;5(1):015014. https://doi.org/10.1088/1758-5082/5/1/015014.
Abdal-Hay A, Hussein KH, Casettari L, Khalil KA, Hamdy AS. Fabrication of novel high performance ductile poly(lactic acid) nanofiber scaffold coated with poly(vinyl alcohol) for tissue engineering applications. Mater Sci Eng C. 2016;60:143-150. https://doi.org/10.1016/j.msec.2015.11.024.
Topsakal A, Uzun M, Ugar G, et al. Development of amoxicillin loaded electrospun polyurethane/chitosan/β -Tricalcium phosphate scaffold for bone tissue regeneration. IEEE Trans Nanobioscience. 2018;17(3):321-328. https://doi.org/10.1109/TNB.2018.2844870.
Waite JH. Mussel power. Nat Mater. 2008;7(1):8-9. https://doi.org/10.1038/nmat2087.
Jiang C, Wang Y, Wang J, Song W, Lu L. Achieving ultrasensitive in vivo detection of bone crack with polydopamine-capsulated surface-enhanced Raman nanoparticle. Biomaterials. 2017;114:54-61. https://doi.org/10.1016/j.biomaterials.2016.11.007.
Lee H, Dellatore SM, Miller WM, Messersmith PB. Mussel-inspired surface chemistry for multifunctional coatings. Science. 2007;318(5849):426-430. https://doi.org/10.1126/science.1147241.
Deng Y, Yang W-Z, Shi D, et al. Bioinspired and osteopromotive polydopamine nanoparticle-incorporated fibrous membranes for robust bone regeneration. NPG Asia Mater. 2019;11(1):39. https://doi.org/10.1038/s41427-019-0139-5.
Liu Y, Ai K, Lu L. Polydopamine and its derivative materials: synthesis and promising applications in energy, environmental, and biomedical fields. Chem Rev. 2014;114(9):5057-5115. https://doi.org/10.1021/cr400407a.
Wang Z, Jiang X, Cheng X, Lau CH, Shao L. Mussel-inspired hybrid coatings that transform membrane hydrophobicity into high hydrophilicity and underwater superoleophobicity for oil-in-water emulsion separation. ACS Appl Mater Interfaces. 2015;7(18):9534-9545. https://doi.org/10.1021/acsami.5b00894.
Ryu J, Ku SH, Lee H, Park CB. Mussel-inspired polydopamine coating as a universal route to hydroxyapatite crystallization. Adv Funct Mater. 2010;20(13):2132-2139. https://doi.org/10.1002/adfm.200902347.
Lynge ME, Ogaki R, Laursen AO, Lovmand J, Sutherland DS, Städler B. Polydopamine/liposome coatings and their interaction with myoblast cells. ACS Appl Mater Interfaces. 2011;3(6):2142-2147. https://doi.org/10.1021/am200358p.
Ghorbani F, Zamanian A, Shams A, Shamoosi A, Aidun A. Fabrication and characterisation of super-paramagnetic responsive PLGA-gelatine-magnetite scaffolds with the unidirectional porous structure: a physicochemical, mechanical, and in vitro evaluation. IET Nanobiotechnol. 2019;13(8):860-867. https://doi.org/10.1049/iet-nbt.2018.5305.
Tian M, Wang Y, Qu L, et al. Electromechanical deformation sensors based on polyurethane/polyaniline electrospinning nanofibrous mats. Synth Met. 2016;219:11-19. https://doi.org/10.1016/j.synthmet.2016.05.005.
Nandagiri VK, Gentile P, Chiono V, et al. Incorporation of PLGA nanoparticles into porous chitosan-gelatin scaffolds: influence on the physical properties and cell behavior. J Mech Behav Biomed Mater. 2011;4(7):1318-1327. https://doi.org/10.1016/j.jmbbm.2011.04.019.
Lizundia E, Mateos P, Vilas JL. Tuneable hydrolytic degradation of poly( l -lactide) scaffolds triggered by ZnO nanoparticles. Mater Sci Eng C. 2017;75:714-720. https://doi.org/10.1016/j.msec.2017.02.104.
Chaisiri O, Chanunpanich N, Hanpanich BS. Polycarpolactone fibers gellation with gelatin ground substance: engineered skin extracellular matrix aims for using as tissue engineering skin. In: Goh J, ed. The 15th International Conference on Biomedical Engineering. IFMBE Proceedings. Vol 43. Cham: Springer; 2014:267-270. https://doi.org/10.1007/978-3-319-02913-9_68.
Ghorbani F, Zamanian A, Kermanian F, Shamoosi A. A bioinspired 3D shape olibanum-collagen-gelatin scaffolds with tunable porous microstructure for efficient neural tissue regeneration. Biotechnol Prog. 2020;36(1):2918. https://doi.org/10.1002/btpr.2918.
Zheng Z, Huang L, Yan L, et al. Polyaniline functionalized graphene nanoelectrodes for the regeneration of PC12 cells via electrical stimulation. Int J Mol Sci. 2019;20(8):2013. https://doi.org/10.3390/ijms20082013.
Bose S, Tarafder S, Bandyopadhyay A. Effect of chemistry on osteogenesis and angiogenesis towards bone tissue engineering using 3D printed scaffolds. Ann Biomed Eng. 2017;45(1):261-272. https://doi.org/10.1007/s10439-016-1646-y.
Kaplan FS, Hayes WC, Keaveny TM, Boskey A, Einhorn TA, Iannotti JP. Form and function of bone. Orthop Basic Sci. 1994;1994:127-185.
Hulbert SF, Young FA, Mathews RS, Klawitter JJ, Talbert CD, Stelling FH. Potential of ceramic materials as permanently implantable skeletal prostheses. J Biomed Mater Res. 1970;4(3):433-456. https://doi.org/10.1002/jbm.820040309.
Yuan H, Kurashina K, de Bruijn JD, Li Y, de Groot K, Zhang X. A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics. Biomaterials. 1999;20(19):1799-1806. https://doi.org/10.1016/S0142-9612(99)00075-7.
Meng ZX, Wang YS, Ma C, Zheng W, Li L, Zheng YF. Electrospinning of PLGA/gelatin randomly-oriented and aligned nanofibers as potential scaffold in tissue engineering. Mater Sci Eng C. 2010;30(8):1204-1210. https://doi.org/10.1016/j.msec.2010.06.018.
Habibi Rad M, Zamanian A, Hadavi SMM, Khanlarkhani A. A two-stage kinetics model for Polydopamine layer growth. Macromol Chem Phys. 2018;219(9):1-6. https://doi.org/10.1002/macp.201700505.
Jiang J, Zhu L, Zhu L, Zhu B, Xu Y. Surface characteristics of a self-polymerized dopamine coating deposited on hydrophobic polymer films. Langmuir. 2011;27(23):14180-14187. https://doi.org/10.1021/la202877k.
Asefnejad A, Khorasani MT, Behnamghader A, Farsadzadeh B, Bonakdar S. Manufacturing of biodegradable polyurethane scaffolds based on polycaprolactone using a phase separation method: physical properties and in vitro assay. Int J Nanomedicine. 2011;6:2375-2384. https://doi.org/10.2147/IJN.S15586.
Valero MF, Pulido JE, Hernández JC, Posada JA, Ramírez A, Cheng C. Preparation and properties of polyurethanes based on Castor oil chemically modified with yucca starch glycoside. Polym Degrad Stab. 2012;95(1):223-244. https://doi.org/10.1177/0095244308091785.
Phua SL, Yang L, Huang S, et al. Shape memory polyurethane with polydopamine-coated nanosheets: simultaneous enhancement of recovery stress and strain recovery ratio and the underlying mechanisms. Eur Polym J. 2014;57:11-21. https://doi.org/10.1016/j.eurpolymj.2014.04.019.
Ghorbani F, Zamanian A, Behnamghader A, Joupari MD. A facile method to synthesize mussel-inspired polydopamine nanospheres as an active template for in situ formation of biomimetic hydroxyapatite. Mater Sci Eng C. 2019;94:729-739. https://doi.org/10.1016/j.msec.2018.10.010.
Alhosseini SN, Moztarzadeh F, Mozafari M, Asgari S, Dodel M, Samadikuchaksaraei A, Kargozar S, Jalali N. Synthesis and characterization of electrospun polyvinyl alcohol nanofibrous scaffolds modified by blending with chitosan for neural tissue engineering. Int J Nanomedicine. 2012;7:25. https://doi.org/10.2147/IJN.S25376.
Rangel-Vázquez NA, Salgado-Delgado R, García-Hernández E, Mendoza-Martínez AM. Characterization of copolymer based in polyurethane and polyaniline (PU/PANI). J Mex Chem Soc. 2009;53(4):248-252.
Zhou C, Han J, Song G, Guo R. Fabrication of polyaniline with hierarchical structures in alkaline solution. Eur Polym J. 2008;44(9):2850-2858. https://doi.org/10.1016/j.eurpolymj.2008.01.025.
Rodrigues PC, Lisboa-filho PN, Mangrich AS, Akcelrud L. Polyaniline/polyurethane networks. II. A spectroscopic study. Polymer. 2005;46:2285-2296. https://doi.org/10.1016/j.polymer.2005.01.020.
Zhang CH, lin YF, Wang WJ, Chen B. Preparation and characterization of hydrophilic modification of polypropylene non-woven fabric by dip-coating PVA (polyvinyl alcohol). Sep Purif Technol. 2008;61(3):276-286. https://doi.org/10.1016/j.seppur.2007.10.019.
O'Brien FJ. Biomaterials & scaffolds for tissue engineering. Mater Today. 2011;14(3):88-95. https://doi.org/10.1016/S1369-7021(11)70058-X.
Story BJ, Wagner WR, Gaisser DM, Cook SD, Rust-Dawicki AM. In vivo performance of a modified CSTi dental implant coating. Int J Oral Maxillofac Implant. 1998;13(6):749-757.
Cullinane DM, Einhorn TA. Biomechanics of bone. Principles of bone biology. USA, MA: Elsevier; 2002:17-32. https://doi.org/10.1016/B978-012098652-1.50104-9.
Linde F, Hvid I. The effect of constraint on the mechanical behaviour of trabecular bone specimens. J Biomech. 1989;22(5):485-490. https://doi.org/10.1016/0021-9290(89)90209-1.
Dunham CE, Takaki SE, Johnson JA, Dunning CE. Mechanical properties of cancellous bone of the distal humerus. Clin Biomech. 2005;20(8):834-838. https://doi.org/10.1016/j.clinbiomech.2005.05.014.
Zhu J, Marchant RE. Design properties of hydrogel tissue-engineering scaffolds. Expert Rev Med Devices. 2011;8(5):607-626. https://doi.org/10.1586/erd.11.27.
Hosseinzadeh S, Mahmoudifard M, Mohamadyar-Toupkanlou F, et al. The nanofibrous PAN-PANi scaffold as an efficient substrate for skeletal muscle differentiation using satellite cells. Bioprocess Biosyst Eng. 2016;39(7):1163-1172. https://doi.org/10.1007/s00449-016-1592-y.
Li Y, Li X, Zhao R, et al. Enhanced adhesion and proliferation of human umbilical vein endothelial cells on conductive PANI-PCL fiber scaffold by electrical stimulation. Mater Sci Eng C. 2017;72:106-112. https://doi.org/10.1016/j.msec.2016.11.052.
Khorshidi S, Karkhaneh A. Particle-coated electrospun scaffold: a semi-conductive drug eluted scaffold with layered fiber/particle arrangement. J Biomed Mater Res, Part A. 2018;106(12):3248-3254. https://doi.org/10.1002/jbm.a.36522.
Chen X, Wu Y, Ranjan VD, Zhang Y. Three-dimensional electrical conductive scaffold from biomaterial-based carbon microfiber sponge with bioinspired coating for cell proliferation and differentiation. Carbon. 2018;134:174-182. https://doi.org/10.1016/j.carbon.2018.03.064.
Xie C, Li P, Han L, et al. Electroresponsive and cell-affinitive polydopamine/polypyrrole composite microcapsules with a dual-function of on-demand drug delivery and cell stimulation for electrical therapy. NPG Asia Mater. 2017;9(3):e358. https://doi.org/10.1038/am.2017.16.
Huang A, Peng X, Geng L, et al. Electrospun poly (butylene succinate)/cellulose nanocrystals bio-nanocomposite scaffolds for tissue engineering: preparation, characterization and in vitro evaluation. Polym Test. 2018;71:101-109. https://doi.org/10.1016/j.polymertesting.2018.08.027.
Ryou M-H, Lee YM, Park J-K, Choi JW. Mussel-inspired polydopamine-treated polyethylene separators for high-power Li-ion batteries. Adv Mater. 2011;23(27):3066-3070. https://doi.org/10.1002/adma.201100303.
Lin C-C, Fu S-J. Osteogenesis of human adipose-derived stem cells on poly(dopamine)-coated electrospun poly(lactic acid) fiber mats. Mater Sci Eng C. 2016;58:254-263. https://doi.org/10.1016/j.msec.2015.08.009.
Li Q, Wei Q, Wu N, Cai Y, Gao W. Structural characterization and dynamic water adsorption of electrospun Polyamide6/ montmorillonite nanofiber. J Appl Polym Sci. 2008;107(6):3535-3540. https://doi.org/10.1002/app.27529.
Tsai WB, Chen WT, Chien HW, Kuo WH, Wang MJ. Poly(dopamine) coating of scaffolds for articular cartilage tissue engineering. Acta Biomater. 2011;7(12):4187-4194. https://doi.org/10.1016/j.actbio.2011.07.024.
Thorvaldsson A, Stenhamre H, Gatenholm P, Walkenstrom P. Electrospinning of highly porous scaffolds for cartilage regeneration. Biomacromolecules. 2008;9(3):1044-1049. https://doi.org/10.1021/bm701225a.
Nwe N, Furuike T, Tamura H. The mechanical and biological properties of chitosan scaffolds for tissue regeneration templates are significantly enhanced by chitosan from Gongronella butleri. Materials. 2009;2(2):374-398. https://doi.org/10.3390/ma2020374.
Brunello G, Sivolella S, Meneghello R, et al. Powder-based 3D printing for bone tissue engineering. Biotechnol Adv. 2016;34(5):740-753. https://doi.org/10.1016/j.biotechadv.2016.03.009.
Marsell R, Einhorn TA. The biology of fracture healing. Injury. 2011;42(6):551-555. https://doi.org/10.1016/j.injury.2011.03.031.
Howard GT. Biodegradation of polyurethane: a review. Int Biodeterior Biodegrad. 2002;49(4):245-252. https://doi.org/10.1016/S0964-8305(02)00051-3.
Ghorbani F, Zamanian A, Behnamghader A, Joupari MD. Microwave-induced rapid formation of biomimetic hydroxyapatite coating on gelatin-siloxane hybrid microspheres in 10X-SBF solution. E-Polymers. 2018;18(3):247-255. https://doi.org/10.1515/epoly-2017-0196.
Li Y, Yang W, Li X, et al. Improving Osteointegration and osteogenesis of three-dimensional porous Ti6Al4V scaffolds by Polydopamine-assisted biomimetic hydroxyapatite coating. ACS Appl Mater Interfaces. 2016;7(10):5715-5724. https://doi.org/10.1021/acsami.5b00331.
Bin YBBB, Pei P, Bin YBBB, et al. Enhance the bioactivity and Osseointegration of the polyethylene-terephthalate-based artificial ligament via poly(dopamine) coating with mesoporous bioactive glass. Adv Eng Mater. 2017;19(5):1600708. https://doi.org/10.1002/adem.201600708.
Meskinfam M, Bertoldi S, Albanese N, et al. Polyurethane foam/nano hydroxyapatite composite as a suitable scaffold for bone tissue regeneration. Mater Sci Eng C. 2018;82:130-140. https://doi.org/10.1016/j.msec.2017.08.064.
Wu X, Liu Y, Li X, et al. Preparation of aligned porous gelatin scaffolds by unidirectional freeze-drying method. Acta Biomater. 2010;6(3):1167-1177. https://doi.org/10.1016/j.actbio.2009.08.041.
Leppik L, Oliveira KMC, Bhavsar MB, Barker JH. Electrical stimulation in bone tissue engineering treatments. Eur J Trauma Emerg Surg. 2020;46(2):231-244. https://doi.org/10.1007/s00068-020-01324-1.
Ding YH, Floren M, Tan W. Mussel-inspired polydopamine for bio-surface functionalization. Biosurf Biotribol. 2016;2(4):121-136. https://doi.org/10.1016/j.bsbt.2016.11.001.
Liu M, Zhou J, Yang Y, Zheng M, Yang J, Tan J. Surface modification of zirconia with polydopamine to enhance fibroblast response and decrease bacterial activity in vitro: a potential technique for soft tissue engineering applications. Colloids Surf B Biointerfaces. 2015;136:74-83. https://doi.org/10.1016/j.colsurfb.2015.06.047.
Kim S, Jang LK, Jang M, Lee S, Hardy JG, Lee JY. Electrically conductive polydopamine-polypyrrole as high performance biomaterials for cell stimulation in vitro and electrical signal recording in vivo. ACS Appl Mater Interfaces. 2018;10(39):33032-33042. https://doi.org/10.1021/acsami.8b11546.
Bidez PR, Li S, MacDiarmid AG, Venancio EC, Wei Y, Lelkes PI. Polyaniline, an electroactive polymer with potential applications in tissue engineering. J Biomater Sci Polym. 2006;17(1):199-212.
LI M, GUO Y, WEI Y, AG MACDIARMID, PI LELKES. Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications. Biomaterials. 2006;27(13):2705-2715. https://doi.org/10.1016/j.biomaterials.2005.11.037.
Saberi A, Jabbari F, Zarrintaj P, Saeb MR, Mozafari M. Electrically conductive materials: opportunities and challenges in tissue engineering. Biomolecules. 2019;9:448. https://doi.org/10.3390/biom9090448.
Roy S, Kuddannaya S, Das T, et al. A novel approach for fabricating highly tunable and fluffy bioinspired 3D poly(vinyl alcohol) (PVA) fiber scaffolds. Nanoscale. 2017;9(21):7081-7093. https://doi.org/10.1039/c7nr00503b.
Ge L, Li Q, Huang Y, et al. Polydopamine-coated paper-stack nanofibrous membranes enhancing adipose stem cells' adhesion and osteogenic differentiation. J Mater Chem B. 2014;2(40):6917-6923. https://doi.org/10.1039/C4TB00570H.
Steeves AJ, Atwal A, Schock SC, Variola F. Evaluation of the direct effects of poly(dopamine) on the in vitro response of human osteoblastic cells. J Mater Chem B. 2016;4(18):3145-3156. https://doi.org/10.1039/C5TB02510A.
Rim NG, Kim SJ, Shin YM, et al. Mussel-inspired surface modification of poly(l-lactide) electrospun fibers for modulation of osteogenic differentiation of human mesenchymal stem cells. Colloids Surf B Biointerfaces. 2012;91(1):189-197. https://doi.org/10.1016/j.colsurfb.2011.10.057.
Hardy JG, Villancio-Wolter MK, Sukhavasi RC, et al. Electrical stimulation of human mesenchymal stem cells on conductive nanofibers enhances their differentiation toward osteogenic outcomes. Macromol Rapid Commun. 2015;36(21):1884-1890. https://doi.org/10.1002/marc.201500233.