Trimethylation of the R5 Silica-Precipitating Peptide Increases Silica Particle Size by Redirecting Orthosilicate Binding.
R5 peptide
particles
silaffin
silica
trimethylation
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
16 11 2020
16 11 2020
Historique:
received:
30
04
2020
revised:
27
06
2020
pubmed:
1
7
2020
medline:
7
7
2021
entrez:
30
6
2020
Statut:
ppublish
Résumé
The unmodified R5 peptide from silaffin in the diatom Cylindrotheca fusiformis rapidly precipitates silica particles from neutral aqueous solutions of orthosilicic acid. A range of post-translational modifications found in R5 contribute toward tailoring silica morphologies in a species-specific manner. We investigated the specific effect of R5 lysine side-chain trimethylation, which adds permanent positive charges, on silica particle formation. Our studies revealed that a doubly trimethylated R5K3,4me3 peptide has reduced maximum activity yet, surprisingly, generates larger silica particles. Molecular dynamics simulations of R5K3,4me3 binding by the precursor orthosilicate anion revealed that orthosilicate preferentially associates with unmodified lysine side-chain amines and the peptide N terminus. Thus, larger silica particles arise from reduced orthosilicate association with trimethylated lysine side chains and their redirection to the N terminus of the R5 peptide.
Identifiants
pubmed: 32596917
doi: 10.1002/cbic.202000264
pmc: PMC8604655
mid: NIHMS1755248
doi:
Substances chimiques
Peptide Fragments
0
Protein Precursors
0
R5 peptide, silaffin-1 precursor
0
Silicic Acid
1343-98-2
Silicon Dioxide
7631-86-9
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3208-3211Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM110430
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD097408
Pays : United States
Informations de copyright
© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
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