Imaging of the Lactate/Pyruvate Ratio Using a Genetically Encoded Förster Resonance Energy Transfer Indicator.

Bacterial Proteins / chemistry Fluorescence Resonance Energy Transfer HEK293 Cells Humans Hydrogen-Ion Concentration Lactic Acid / metabolism Models, Molecular Molecular Imaging / methods NAD / metabolism Protein Conformation Pyruvic Acid / metabolism

Journal

Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536

Informations de publication

Date de publication:
04 08 2020
Historique:
pubmed: 1 7 2020
medline: 17 2 2021
entrez: 1 7 2020
Statut: ppublish

Résumé

The ratio between the cytosolic concentrations of lactate and pyruvate is a direct readout of the balance between glycolysis and mitochondrial oxidative metabolism. Current approaches do not allow detection of the lactate/pyruvate ratio in a single readout with high spatial/temporal resolution in living systems. Using a Förster resonance energy transfer (FRET)-based screening strategy, we found that the orphan transcriptional factor

Identifiants

DOI: 10.1021/acs.analchem.0c01741 PMID: 32600029
pubmed: 32600029
doi: 10.1021/acs.analchem.0c01741
doi:

Substances chimiques

Bacterial Proteins 0
NAD 0U46U6E8UK
Lactic Acid 33X04XA5AT
Pyruvic Acid 8558G7RUTR

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

10643-10650

Auteurs

Alex Galaz (A)

Centro de Estudios Cientı́ficos (CECs), Avenida Arturo Prat 514, Valdivia 5110466, Chile.

Francisca Cortés-Molina (F)

Centro de Estudios Cientı́ficos (CECs), Avenida Arturo Prat 514, Valdivia 5110466, Chile.

Robinson Arce-Molina (R)

Centro de Estudios Cientı́ficos (CECs), Avenida Arturo Prat 514, Valdivia 5110466, Chile.

Ignacio Romero-Gómez (I)

Centro de Estudios Cientı́ficos (CECs), Avenida Arturo Prat 514, Valdivia 5110466, Chile.

Gonzalo Antonio Mardones (GA)

Instituto de Fisiologı́a, Facultad de Medicina, Universidad Austral de Chile (UACh), Isla Teja s/n, Valdivia 5110566, Chile.

L Felipe Barros (L)

Centro de Estudios Cientı́ficos (CECs), Avenida Arturo Prat 514, Valdivia 5110466, Chile.

Alejandro San Martín (A)

Centro de Estudios Cientı́ficos (CECs), Avenida Arturo Prat 514, Valdivia 5110466, Chile.
ORCID: 0000-0002-5608-5117

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Imaging of the Lactate/Pyruvate Ratio Using a...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Transfert d'énergie par résonance de fluorescence › Imaging of the Lactate/Pyruvate Ratio Using a...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Imaging of the Lactate/Pyruvate Ratio Using a...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Imaging of the Lactate/Pyruvate Ratio Using a...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Imaging of the Lactate/Pyruvate Ratio Using a...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Hydroxyacides › Lactates › Acide lactique › Imaging of the Lactate/Pyruvate Ratio Using a...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Imaging of the Lactate/Pyruvate Ratio Using a...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Imagerie moléculaire › Imaging of the Lactate/Pyruvate Ratio Using a...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › NAD › Imaging of the Lactate/Pyruvate Ratio Using a...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Imaging of the Lactate/Pyruvate Ratio Using a...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Cétoacides › Pyruvates › Acide pyruvique › Imaging of the Lactate/Pyruvate Ratio Using a...