Activation of pro- and anti-inflammatory responses in lung tissue injury during the acute phase of PRRSV-1 infection with the virulent strain Lena.
Acute Disease
Age Factors
Animals
Antibodies, Viral
/ blood
Bronchopneumonia
/ immunology
Cytokines
/ blood
Female
Inflammation
/ immunology
Lung
/ immunology
Macrophages, Alveolar
/ immunology
Male
Porcine Reproductive and Respiratory Syndrome
/ immunology
Porcine respiratory and reproductive syndrome virus
/ genetics
Swine
Viral Load
Viremia
/ immunology
Virulence
Inflammatory response
Lung lesion
PRRSV-1
Virulent strains
Journal
Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
08
04
2020
revised:
29
05
2020
accepted:
31
05
2020
entrez:
2
7
2020
pubmed:
2
7
2020
medline:
19
3
2021
Statut:
ppublish
Résumé
Porcine reproductive and respiratory syndrome virus (PRRSV) plays a key role in porcine respiratory disease complex modulating the host immune response and favouring secondary bacterial infections. Pulmonary alveolar macrophages (PAMs) are the main cells supporting PRRSV replication, with CD163 as the essential receptor for viral infection. Although interstitial pneumonia is by far the representative lung lesion, suppurative bronchopneumonia is described for PRRSV virulent strains. This research explores the role of several immune markers potentially involved in the regulation of the inflammatory response and sensitisation of lung to secondary bacterial infections by PRRSV-1 strains of different virulence. Conventional pigs were intranasally inoculated with the virulent subtype 3 Lena strain or the low virulent subtype 1 3249 strain and euthanised at 1, 3, 6 and 8 dpi. Lena-infected pigs exhibited more severe clinical signs, macroscopic lung score and viraemia associated with an increase of IL-6 and IFN-γ in sera compared to 3249-infected pigs. Extensive areas of lung consolidation corresponding with suppurative bronchopneumonia were observed in Lena-infected pigs. Lung viral load and PRRSV-N-protein
Identifiants
pubmed: 32605751
pii: S0378-1135(20)30433-8
doi: 10.1016/j.vetmic.2020.108744
pmc: PMC7265841
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Cytokines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108744Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no competing interest.
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