One-Step Bacterial Artificial Chromosome (BAC) Modification: Preparation of the A Homology Arm (A-Box).
Journal
Cold Spring Harbor protocols
ISSN: 1559-6095
Titre abrégé: Cold Spring Harb Protoc
Pays: United States
ID NLM: 101524530
Informations de publication
Date de publication:
01 07 2020
01 07 2020
Historique:
entrez:
3
7
2020
pubmed:
3
7
2020
medline:
8
6
2021
Statut:
epublish
Résumé
The one-step approach to bacterial artificial chromosome (BAC) modification requires that only one homology arm be cloned into the shuttle vector (in the example presented here, we use the "A-box"). The homology arm, which in this case lies upstream of the ATG start codon, is amplified by polymerase chain reaction (PCR) using purified BAC DNA as template. The resulting amplification product is then digested with the appropriate restriction endonuclease to render it suitable for cloning into the shuttle vector.
Identifiants
pubmed: 32611777
pii: 2020/7/pdb.prot098103
doi: 10.1101/pdb.prot098103
doi:
Substances chimiques
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
098103Informations de copyright
© 2020 Cold Spring Harbor Laboratory Press.