Establishment of a Simple Method for Inducing Neuronal Differentiation of P19 EC Cells without Embryoid Body Formation and Analysis of the Role of Histone Deacetylase 8 Activity in This Differentiation.
N2B27 medium
P19 pluripotent embryonic carcinoma stem cell
histone deacetylase 8
monolayer culture
neuronal differentiation
Journal
Biological & pharmaceutical bulletin
ISSN: 1347-5215
Titre abrégé: Biol Pharm Bull
Pays: Japan
ID NLM: 9311984
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
3
7
2020
pubmed:
3
7
2020
medline:
1
1
2021
Statut:
ppublish
Résumé
P19 pluripotent embryonic carcinoma (EC) stem cells are derived from pluripotent germ cell tumours and can differentiate into three germ layers. Treatment of these cells in suspension culture with retinoic acid induces their differentiation into neurons and glial cells. Hence, these cells are an excellent in vitro model to study the transition from the upper blastoderm to the neuroectoderm. However, because of the complex nature of the techniques involved, the results are highly dependent on the skills of the experimenter. Herein, we developed a simple method to induce neuronal differentiation of adherent P19 EC cells in TaKaRa NDiff
Identifiants
pubmed: 32612072
doi: 10.1248/bpb.b20-00091
doi:
Substances chimiques
Histone Deacetylase Inhibitors
0
Hydroxamic Acids
0
N-hydroxy-3-(1-(phenylthio)methyl-1H-1,2,3-triazol-4-yl)benzamide
0
Triazoles
0
HDAC8 protein, mouse
EC 3.5.1.98
Histone Deacetylases
EC 3.5.1.98
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM