Quantitative Imaging of B1 Cyclin Expression Across the Cell Cycle Using Green Fluorescent Protein Tagging and Epifluorescence.
U-2 OS cells
cyclin B1-eGFP
fluorescence microscopy
fluorophore concentration
Journal
Cytometry. Part A : the journal of the International Society for Analytical Cytology
ISSN: 1552-4930
Titre abrégé: Cytometry A
Pays: United States
ID NLM: 101235694
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
20
11
2019
revised:
21
04
2020
accepted:
28
04
2020
pubmed:
3
7
2020
medline:
30
7
2021
entrez:
3
7
2020
Statut:
ppublish
Résumé
In this article, we report the number of cyclin B1 proteins tagged with enhanced green fluorescent protein (eGFP) in fixed U-2 OS cells across the cell cycle. We use a quantitative analysis of epifluorescence to determine the number of eGFP molecules in a nondestructive way, and integrated over the cell we find 10
Identifiants
pubmed: 32613720
doi: 10.1002/cyto.a.24038
doi:
Substances chimiques
Cyclin B1
0
Cyclins
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1066-1072Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/H003746/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M000249/1
Pays : United Kingdom
Informations de copyright
© 2020 The Authors. Cytometry Part A published by Wiley Periodicals LLC. on behalf of International Society for Advancement of Cytometry.
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