Silencing RNF13 Alleviates Parkinson's Disease - Like Problems in Mouse Models by Regulating the Endoplasmic Reticulum Stress-Mediated IRE1α-TRAF2-ASK1-JNK Pathway.
Animals
Apoptosis
Calcium
/ metabolism
Cell Line, Tumor
Dopaminergic Neurons
/ metabolism
Endoplasmic Reticulum Stress
Gene Silencing
Humans
MAP Kinase Kinase 4
/ metabolism
MAP Kinase Kinase Kinase 5
/ metabolism
MPTP Poisoning
/ genetics
Male
Membrane Proteins
/ metabolism
Mice
Mice, Inbred C57BL
Movement
Protein Serine-Threonine Kinases
/ metabolism
Signal Transduction
TNF Receptor-Associated Factor 2
/ metabolism
Ubiquitin-Protein Ligases
/ genetics
Endoplasmic reticulum stress
IRE1α-TRAF2-ASK1-JNK pathway
Parkinson’s disease
RNF13
Journal
Journal of molecular neuroscience : MN
ISSN: 1559-1166
Titre abrégé: J Mol Neurosci
Pays: United States
ID NLM: 9002991
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
06
03
2020
accepted:
15
05
2020
pubmed:
4
7
2020
medline:
20
8
2021
entrez:
4
7
2020
Statut:
ppublish
Résumé
The objective of this study was to understand if RNF13 can affect Parkinson's disease (PD) model mice by modulating the endoplasmic reticulum stress (ERS)-mediated IRE1α-TRAF2-ASK1-JNK pathway. C57BL/6 mice injected with MPTP to establish PD mice models were divided into Control, MPTP, MPTP + sh-RNF13, and MPTP + sh-NC groups. Rotarod, balance beam, and open-field tests were used to assess the behavioral changes of experimental mice. Immunofluorescence assay was used to determine TH-positive expression in substantia nigra, TUNEL staining to detect apoptosis, and Western blotting to measure the expression of IRE1α-TRAF2-ASK1-JNK pathway. Besides, SH-SY5Y cells treated with MPP
Identifiants
pubmed: 32617872
doi: 10.1007/s12031-020-01599-4
pii: 10.1007/s12031-020-01599-4
doi:
Substances chimiques
Membrane Proteins
0
TNF Receptor-Associated Factor 2
0
TRAF2 protein, mouse
0
RNF13 protein, mouse
EC 2.3.2.27
Ubiquitin-Protein Ligases
EC 2.3.2.27
Ern2 protein, mouse
EC 2.7.1.-
Protein Serine-Threonine Kinases
EC 2.7.11.1
MAP Kinase Kinase Kinase 5
EC 2.7.11.25
Map3k5 protein, mouse
EC 2.7.11.25
MAP Kinase Kinase 4
EC 2.7.12.2
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1977-1986Subventions
Organisme : National Natural Science Foundation and Youth Science Fund Project
ID : No. 81703853
Commentaires et corrections
Type : ErratumIn