Development of NSAID-loaded nano-composite scaffolds for skin tissue engineering applications.
Animals
Anti-Inflammatory Agents, Non-Steroidal
/ administration & dosage
Biomimetics
Cattle
Ibuprofen
/ administration & dosage
Mesenchymal Stem Cells
Microscopy, Electron, Scanning
Nanostructures
/ toxicity
Porosity
Rats
Rats, Sprague-Dawley
Skin Diseases
/ therapy
Skin, Artificial
Spectroscopy, Fourier Transform Infrared
Tissue Engineering
/ methods
Tissue Scaffolds
Wound Healing
/ drug effects
3-D scaffolds
ASCs proliferation
biocompatibility
pain management
wound healing
Journal
Journal of biomedical materials research. Part B, Applied biomaterials
ISSN: 1552-4981
Titre abrégé: J Biomed Mater Res B Appl Biomater
Pays: United States
ID NLM: 101234238
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
27
12
2019
revised:
10
04
2020
accepted:
29
04
2020
pubmed:
4
7
2020
medline:
9
11
2021
entrez:
4
7
2020
Statut:
ppublish
Résumé
Scar free healing together with pain management is one of the major considerations in full thickness wound healing. Extensive wounds take longer to heal without any clinical intervention and, hence, need natural or artificial extracellular matrix support for quick skin regeneration. To address these issues, medicated 3D porous biomimetic scaffolds were developed with a unique combination of biopolymers, that is, chitosan, sodium alginate, and elastin, supplemented with a non-steroidal anti-inflammatory drug (NSAID). Scaffolds were physically characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), swelling ratio analysis, and degradation studies. Findings of the performed analyses proved that these skin substitutes suitable for skin tissue engineering applications attributable to their nano-microporous structures (pore size in range of 0.085-256 μm) allowing cell infiltration and high-water absorption capacity for management of wound exudates. Optimal dose of the loaded ibuprofen was estimated by evaluating effect of variable concentrations of ibuprofen (control, ILM-10, ILM-15, and ILM-20) on adipose tissue-derived mesenchymal stem cells (ASCs) proliferation rate. Out of all experimental groups, ILM-20 constructs were found to accelerate the proliferation rate of seeded ASCs confirming their non-cytotoxic characteristics as well potential to be used for translational scaffold-based therapies.
Substances chimiques
Anti-Inflammatory Agents, Non-Steroidal
0
Ibuprofen
WK2XYI10QM
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3064-3075Informations de copyright
© 2020 Wiley Periodicals LLC.
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