Template-based peptide modeling for celiac risk assessment of newly expressed proteins in GM crops.
Celiac disease
GM crops
Peptide modeling
Journal
Regulatory toxicology and pharmacology : RTP
ISSN: 1096-0295
Titre abrégé: Regul Toxicol Pharmacol
Pays: Netherlands
ID NLM: 8214983
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
12
03
2020
revised:
07
06
2020
accepted:
16
06
2020
pubmed:
4
7
2020
medline:
10
4
2021
entrez:
4
7
2020
Statut:
ppublish
Résumé
Newly expressed proteins in genetically modified (GM) crops are subject to celiac disease risk assessment according to EFSA guidelines. Amino acid identity matches between short peptides (9aa) and known celiac restricted epitopes are required to be further evaluated through peptide modeling; however, validated methods and criteria are not yet available. In this investigation, several structures of HLA-DQ2.5/peptide/TCR (T-cell receptor) complexes were analyzed and two template-based peptide molding software packages were evaluated using various peptides including ones not associated with celiac disease. Structural characterization indicates that residues at P(position)1, P2, P5, P8, and P9 in the 9aa restricted epitopes also contribute to the binding of celiac peptides to the HLA-DQ2.5 antigen in addition to the presence of the motif Q/EX
Identifiants
pubmed: 32619636
pii: S0273-2300(20)30141-0
doi: 10.1016/j.yrtph.2020.104715
pii:
doi:
Substances chimiques
Epitopes
0
HLA-DQ Antigens
0
HLA-DQ2 antigen
0
Peptides
0
Plant Proteins
0
Receptors, Antigen, T-Cell
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104715Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.