A convenient and rapid method for detecting d-glucose in honey used smartphone.
Biosensor
Color reacting
Enzyme
Journal
Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639
Informations de publication
Date de publication:
30 Nov 2020
30 Nov 2020
Historique:
received:
12
11
2019
revised:
20
03
2020
accepted:
12
06
2020
pubmed:
4
7
2020
medline:
21
10
2020
entrez:
4
7
2020
Statut:
ppublish
Résumé
Information concerning food composition, including information on its glucose content, is essential for modern food industry due to greater consumer awareness and expectations. In this work, the gene encoding d-glucose dehydrogenase (GDH) from Bacillus Natto was expressed in Escherichia coli BL21(DE3) firstly. Ni-IDA column was used for the purification of GDH. Then, the purified GDH was used to construct a color system with stable and effective measurement of concentration of d-glucose. The smart phone photographing and the software Microsoft Photoshop have been used in the system for determination of the color. The enzymatic analysis system can detect the concentration of d-glucose from 5 mM to 40 mM, and other various sugars has no interference to the system. The system was used to quantitatively detect the concentration of d-glucose in honey. The system can be used for convenient and rapid detection of d-glucose in food, especially for large numbers of samples.
Identifiants
pubmed: 32619908
pii: S0308-8146(20)31210-3
doi: 10.1016/j.foodchem.2020.127348
pii:
doi:
Substances chimiques
Glucose 1-Dehydrogenase
EC 1.1.1.47
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
127348Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.