Wnt-controlled sphingolipids modulate Anthrax Toxin Receptor palmitoylation to regulate oriented mitosis in zebrafish.
Amino Acid Sequence
Animals
Asymmetric Cell Division
/ genetics
Embryo, Nonmammalian
/ cytology
Gastrulation
Gene Expression Regulation, Developmental
Germ Layers
/ cytology
Lipoylation
Mitosis
Neural Tube
/ cytology
Receptors, Peptide
/ genetics
Sequence Homology, Amino Acid
Serine C-Palmitoyltransferase
/ genetics
Sphingolipids
/ metabolism
Spindle Apparatus
/ metabolism
Wnt Signaling Pathway
Zebrafish
Zebrafish Proteins
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
03 07 2020
03 07 2020
Historique:
received:
07
06
2019
accepted:
17
06
2020
entrez:
5
7
2020
pubmed:
6
7
2020
medline:
1
9
2020
Statut:
epublish
Résumé
Oriented cell division is a fundamental mechanism to control asymmetric stem cell division, neural tube elongation and body axis extension, among other processes. During zebrafish gastrulation, when the body axis extends, dorsal epiblast cells display divisions that are robustly oriented along the animal-vegetal embryonic axis. Here, we use a combination of lipidomics, metabolic tracer analysis and quantitative image analysis to show that sphingolipids mediate spindle positioning during oriented division of epiblast cells. We identify the Wnt signaling as a regulator of sphingolipid synthesis that mediates the activity of serine palmitoyltransferase (SPT), the first and rate-limiting enzyme in sphingolipid production. Sphingolipids determine the palmitoylation state of the Anthrax receptor, which then positions the mitotic spindle of dividing epiblast cells. Our data show how Wnt signaling mediates sphingolipid-dependent oriented division and how sphingolipids determine Anthrax receptor palmitoylation, which ultimately controls the activation of Diaphanous to mediate spindle rotation and oriented mitosis.
Identifiants
pubmed: 32620775
doi: 10.1038/s41467-020-17196-3
pii: 10.1038/s41467-020-17196-3
pmc: PMC7335183
doi:
Substances chimiques
Receptors, Peptide
0
Sphingolipids
0
Zebrafish Proteins
0
anthrax toxin receptors
0
Serine C-Palmitoyltransferase
EC 2.3.1.50
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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