"
Sertoli cells
alpha follitropin
beta follitropin
proteomic analysis
urofollitropin
Journal
Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782
Informations de publication
Date de publication:
2020
2020
Historique:
received:
23
03
2020
accepted:
19
05
2020
entrez:
7
7
2020
pubmed:
7
7
2020
medline:
14
5
2021
Statut:
epublish
Résumé
Follicle-stimulating hormone (FSH), a major regulator of spermatogenesis, has a crucial function in the development and function of the testis and it is extensively given as a fertility treatment to stimulate spermatogenesis. We analyzed the effects of different FSH preparations (α-follitropin, β-follitropin, and urofollitropin) in combination with testosterone on porcine pre-pubertal Sertoli cells. To study the effect of the different FSH treatments in the Sertoli cell function we performed Real Time PCR analysis of AMH, inhibin B, and FSH-r, an ELISA assay for AMH and inhibin B, and a high-throughput comparative proteomic analysis. We verified that all three preparations induced a reduction of AMH in terms of mRNA and secreted proteins, and an increase of inhibin B in terms of mRNA in all the FSH formulations, while solely α-follitropin produced an increase of secreted inhibin B in the culture medium. Comparative proteomic analysis of the three FSH preparations identified 46 proteins, 11 up-regulated and 2 down-regulated. Surprisingly, the combination of testosterone with β-follitropin specifically induced an up-regulation of eight specific secreted proteins. Our study, showing that the three different FSH preparations induce different effects, could offer the opportunity to shed light inside new applications to a personalized reproductive medicine.
Identifiants
pubmed: 32625170
doi: 10.3389/fendo.2020.00401
pmc: PMC7314925
doi:
Substances chimiques
Testosterone
3XMK78S47O
Follicle Stimulating Hormone
9002-68-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
401Informations de copyright
Copyright © 2020 Arato, Grande, Barrachina, Bellucci, Lilli, Jodar, Aglietti, Mancini, Vincenzoni, Pontecorvi, Calafiore, Oliva, Luca, Mancuso and Milardi.
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