Microglia phagocytose myelin sheaths to modify developmental myelination.
Journal
Nature neuroscience
ISSN: 1546-1726
Titre abrégé: Nat Neurosci
Pays: United States
ID NLM: 9809671
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
01
12
2019
accepted:
13
05
2020
pubmed:
8
7
2020
medline:
1
12
2020
entrez:
8
7
2020
Statut:
ppublish
Résumé
During development, oligodendrocytes contact and wrap neuronal axons with myelin. Similarly to neurons and synapses, excess myelin sheaths are produced and selectively eliminated, but how elimination occurs is unknown. Microglia, the resident immune cells of the central nervous system, engulf surplus neurons and synapses. To determine whether microglia also prune myelin sheaths, we used zebrafish to visualize and manipulate interactions between microglia, oligodendrocytes, and neurons during development. We found that microglia closely associate with oligodendrocytes and specifically phagocytose myelin sheaths. By using a combination of optical, genetic, chemogenetic, and behavioral approaches, we reveal that neuronal activity bidirectionally balances microglial association with neuronal cell bodies and myelin phagocytosis in the optic tectum. Furthermore, multiple strategies to deplete microglia resulted in oligodendrocytes maintaining excessive and ectopic myelin. Our work reveals a neuronal activity-regulated role for microglia in modifying developmental myelin targeting by oligodendrocytes.
Identifiants
pubmed: 32632287
doi: 10.1038/s41593-020-0654-2
pii: 10.1038/s41593-020-0654-2
pmc: PMC7483351
mid: NIHMS1594382
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1055-1066Subventions
Organisme : NINDS NIH HHS
ID : P30 NS048154
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS095679
Pays : United States
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