Imaging Plant Cells by High-Pressure Freezing and Serial block-face scanning electron microscopy.

Arabidopsis / ultrastructure Freeze Substitution Microscopy, Electron, Scanning / instrumentation Microtomy / instrumentation
High-pressure freezing Serial block-face electron microscopy Three-dimensional electron microscopy

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2020
Historique:
entrez: 8 7 2020
pubmed: 8 7 2020
medline: 19 3 2021
Statut: ppublish

Résumé

This chapter describes methods to enhanced contrast of plant material processed by high-pressure freezing and freeze substitution for improved visualization by serial block-face scanning electron microscopy (SBEM). The contrast enhancing steps are based on a protocol involving the sequential incubation of samples in heavy metals and sodium thiocarbohydrazide (OTO staining). We also describe the pipeline for imaging plant tissues in a commercial SBEM system (Gatan 3View

Identifiants

DOI: 10.1007/978-1-0716-0767-1_7 PMID: 32632806
pubmed: 32632806
doi: 10.1007/978-1-0716-0767-1_7
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

69-81

Auteurs

Kirk Czymmek (K)

Advanced Bioimaging Laboratory, Donald Danforth Plant Science Center, St. Louis, MO, USA.

Abhilash Sawant (A)

Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, WI, USA.
Department of Neuroscience, University of Wisconsin-Madison, Madison, WI, USA.

Kaija Goodman (K)

Department of Botany, University of Wisconsin-Madison, Madison, WI, USA.
Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison, Madison, WI, USA.

Janice Pennington (J)

Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison, Madison, WI, USA.

Pal Pedersen (P)

Carl Zeiss Microscopy, LLC, White Plains, NY, USA.

Mrinalini Hoon (M)

Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, WI, USA.
McPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI, USA.

Marisa S Otegui (MS)

Department of Botany, University of Wisconsin-Madison, Madison, WI, USA. otegui@wisc.edu.
Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison, Madison, WI, USA. otegui@wisc.edu.

Articles similaires

A cytoplasmic osmosensing mechanism mediated by molecular crowding-sensitive DCP5.

Zhenyu Wang, Qiuhua Yang, Dan Zhang et al.
1.00
Arabidopsis Arabidopsis Proteins Osmotic Pressure Cytoplasm RNA, Messenger

Exploring the complexity of genome size reduction in angiosperms.

Akihiro Ezoe, Motoaki Seki
1.00
Genome Size Genome, Plant Magnoliopsida Evolution, Molecular Arabidopsis

Artificial selection of two antagonistic E3 ubiquitin ligases finetunes soybean photoperiod adaptation and grain yield.

Fan Wang, Shuangrong Liu, Haiyang Li et al.
1.00
Glycine max Photoperiod Ubiquitin-Protein Ligases Flowers Gene Expression Regulation, Plant

Plant plasmodesmata bridges form through ER-dependent incomplete cytokinesis.

Ziqiang P Li, Hortense Moreau, Jules D Petit et al.
1.00
Plasmodesmata Endoplasmic Reticulum Arabidopsis Cytokinesis Arabidopsis Proteins

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › Imaging Plant Cells by High-Pressure Freezing...
questionsmedicales.fr › Techniques d'investigation › Conservation biologique › Cryoconservation › Lyophilisation › Congélation-dissolution › Imaging Plant Cells by High-Pressure Freezing...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à balayage › Imaging Plant Cells by High-Pressure Freezing...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Microtomie › Imaging Plant Cells by High-Pressure Freezing...