An update on the origin of SARS-CoV-2: Despite closest identity, bat (RaTG13) and pangolin derived coronaviruses varied in the critical binding site and O-linked glycan residues.
Animals
Binding Sites
China
Chiroptera
/ virology
Communicable Disease Control
Coronavirus
/ genetics
Coronavirus Envelope Proteins
/ chemistry
Gene Expression Regulation, Viral
Host Specificity
Humans
Models, Molecular
Pangolins
/ virology
Phylogeny
Polysaccharides
/ chemistry
Protein Conformation
SARS-CoV-2
/ genetics
Spike Glycoprotein, Coronavirus
/ chemistry
COVID-19
RaTg13
SARS-CoV-2
intermediate host
pangolins
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
14
05
2020
revised:
23
06
2020
accepted:
04
07
2020
pubmed:
8
7
2020
medline:
10
3
2021
entrez:
8
7
2020
Statut:
ppublish
Résumé
The initial cases of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) occurred in Wuhan, China, in December 2019 and swept the world by 23 June 2020 with 8 993 659 active cases, 469 587 deaths across 216 countries, areas or territories. This strongly implies global transmission occurred before the lockdown of China. However, the initial source's transmission routes of SARS-CoV-2 remain obscure and controversial. Research data suggest bat (RaTG13) and pangolin carried CoV were the proximal source of SARS-CoV-2. In this study, we used systematic phylogenetic analysis of Coronavirinae subfamily along with wild type human SARS-CoV, MERS-CoV, and SARS-CoV-2 strains. The key residues of the receptor-binding domain (RBD) and O-linked glycan were compared. SARS-CoV-2 strains were clustered with RaTG13 (97.41% identity), Pangolin-CoV (92.22% identity) and Bat-SL-CoV (80.36% identity), forms a new clade-2 in lineage B of beta-CoV. The alignments of RBD contact residues to ACE2 justified? Those SARS-CoV-2 strains sequences were 100% identical by each other, significantly varied in RaTG13 and pangolin-CoV. SARS-CoV-2 has a polybasic cleavage site with an inserted sequence of PRRA compared to RaTG13 and only PRR to pangolin. Only serine (Ser) in pangolin and both threonine (Thr) and serine (Ser) O-linked glycans were seen in RaTG13, suggesting that a detailed study needed in pangolin (Manis javanica) and bat (Rhinolophus affinis) related CoV.
Identifiants
pubmed: 32633815
doi: 10.1002/jmv.26261
pmc: PMC7361880
doi:
Substances chimiques
Coronavirus Envelope Proteins
0
Polysaccharides
0
Spike Glycoprotein, Coronavirus
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
499-505Informations de copyright
© 2020 Wiley Periodicals LLC.
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