A Non-Replicative Role of the 3' Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation.

3' Untranslated Regions / genetics 5' Untranslated Regions / genetics Cell Line DNA-Directed DNA Polymerase / metabolism Dengue / virology Dengue Virus / enzymology Genome, Viral Humans Membranes Nucleic Acid Conformation Organelles / metabolism Plasmids / genetics Polyproteins / metabolism RNA, Viral / genetics Virus Replication / genetics Zika Virus / genetics
flavivirus membrane invagination membranous organelle organelle biogenesis replication complex replication organelle vesicle packet viral replicase

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
07 07 2020
Historique:
received: 27 11 2019
revised: 11 04 2020
accepted: 12 06 2020
entrez: 9 7 2020
pubmed: 9 7 2020
medline: 29 4 2021
Statut: ppublish

Résumé

Dengue virus (DENV) and Zika virus (ZIKV), members of the Flavivirus genus, rearrange endoplasmic reticulum membranes to induce invaginations known as vesicle packets (VPs), which are the assumed sites for viral RNA replication. Mechanistic information on VP biogenesis has so far been difficult to attain due to the necessity of studying their formation under conditions of viral replication, where perturbations reducing replication will inevitably impact VP formation. Here, we report a replication-independent expression system, designated pIRO (plasmid-induced replication organelle formation) that induces bona fide DENV and ZIKV VPs that are morphologically indistinguishable from those in infected cells. Using this system, we demonstrate that sequences in the 3' terminal RNA region of the DENV, but not the ZIKV genome, contribute to VP formation in a non-replicative manner. These results validate the pIRO system that opens avenues for mechanistically dissecting virus replication from membrane reorganization.

Identifiants

DOI: 10.1016/j.celrep.2020.107859 PMID: 32640225 PMC: PMC7351112
pubmed: 32640225
pii: S2211-1247(20)30840-8
doi: 10.1016/j.celrep.2020.107859
pmc: PMC7351112
pii:
doi:

Substances chimiques

3' Untranslated Regions 0
5' Untranslated Regions 0
Polyproteins 0
RNA, Viral 0
DNA replicase EC 2.7.7.-
DNA-Directed DNA Polymerase EC 2.7.7.7

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

107859

Informations de copyright

Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests The authors declare no competing interests.

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Auteurs

Berati Cerikan (B)

Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany.

Sarah Goellner (S)

Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany.

Christopher John Neufeldt (CJ)

Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany.

Uta Haselmann (U)

Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany.

Klaas Mulder (K)

Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany.

Laurent Chatel-Chaix (L)

Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany.

Mirko Cortese (M)

Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany.

Ralf Bartenschlager (R)

Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany; German Center for Infection Research (DZIF), Heidelberg Partner Site, University, 69120 Heidelberg, Germany. Electronic address: ralf.bartenschlager@med.uni-heidelberg.de.

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Régions non traduites › Régions 3' non traduites › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Régions non traduites › Régions 5' non traduites › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › DNA nucleotidyltransferases › DNA-directed DNA polymerase › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Infection › Maladies virales › Infections à virus à ARN › Fièvres hémorragiques virales › Dengue › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Flaviviridae › Flavivirus › Virus de la dengue › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome viral › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Tissus › Membranes › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Polyprotéines › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN viral › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › A Non-Replicative Role of the 3' Terminal...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Flaviviridae › Flavivirus › Virus Zika › A Non-Replicative Role of the 3' Terminal...