Optimized method for extraction of exosomes from human primary muscle cells.
Extracellular vesicle
Muscle exosome extraction in vitro
Muscle secretome
Journal
Skeletal muscle
ISSN: 2044-5040
Titre abrégé: Skelet Muscle
Pays: England
ID NLM: 101561193
Informations de publication
Date de publication:
08 07 2020
08 07 2020
Historique:
received:
05
04
2020
accepted:
22
06
2020
entrez:
10
7
2020
pubmed:
10
7
2020
medline:
29
7
2021
Statut:
epublish
Résumé
Skeletal muscle is increasingly considered an endocrine organ secreting myokines and extracellular vesicles (exosomes and microvesicles), which can affect physiological changes with an impact on different pathological conditions, including regenerative processes, aging, and myopathies. Primary human myoblasts are an essential tool to study the muscle vesicle secretome. Since their differentiation in conditioned media does not induce any signs of cell death or cell stress, artefactual effects from those processes are unlikely. However, adult human primary myoblasts senesce in long-term tissue culture, so a major technical challenge is posed by the need to avoid artefactual effects resulting from pre-senescent changes. Since these cells should be studied within a strictly controlled pre-senescent division count (<21 divisions), and yields of myoblasts per muscle biopsy are low, it is difficult or impossible to amplify sufficiently large cell numbers (some 250 × 10
Identifiants
pubmed: 32641118
doi: 10.1186/s13395-020-00238-1
pii: 10.1186/s13395-020-00238-1
pmc: PMC7341622
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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