Performance of PCR/Electrospray Ionization-Mass Spectrometry on Whole Blood for Detection of Bloodstream Microorganisms in Patients with Suspected Sepsis.

Blood Culture Europe Humans Polymerase Chain Reaction Sepsis / diagnosis Spectrometry, Mass, Electrospray Ionization
PCR/ESI-MS bacteremia direct detection sepsis

Journal

Journal of clinical microbiology
ISSN: 1098-660X
Titre abrégé: J Clin Microbiol
Pays: United States
ID NLM: 7505564

Informations de publication

Date de publication:
24 08 2020
Historique:
received: 06 11 2019
accepted: 01 07 2020
pubmed: 10 7 2020
medline: 24 6 2021
entrez: 10 7 2020
Statut: epublish

Résumé

Blood culture (BC) often fails to detect bloodstream microorganisms in sepsis. However, molecular diagnostics hold great potential. The molecular method PCR/electrospray ionization-mass spectrometry (PCR/ESI-MS) can detect DNA from hundreds of different microorganisms in whole blood. The aim of the present study was to evaluate the performance of this method in a multicenter study including 16 teaching hospitals in the United States (

Identifiants

DOI: 10.1128/JCM.01860-19 PMID: 32641399 PMC: PMC7448645
pubmed: 32641399
pii: JCM.01860-19
doi: 10.1128/JCM.01860-19
pmc: PMC7448645
pii:
doi:

Types de publication

Journal Article Multicenter Study Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2020 Strålin et al.

Références

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Auteurs

Kristoffer Strålin (K)

Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden kristoffer.stralin@ki.se.
Department of Medicine Huddinge, Karolinska Institutet, Stockholm, Sweden.
ORCID: 0000-0002-8801-3169

Richard E Rothman (RE)

Department of Emergency Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Volkan Özenci (V)

Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.
Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.

Kieron Barkataki (K)

Department of Emergency Medicine, Kern Medical Center, Bakersfield, California, USA.

David Brealey (D)

Division of Critical Care, NIHR Biomedical Research Centre at University of College London Hospitals NHS Foundation Trust, London, United Kingdom.

Neelam Dhiman (N)

Molecular Infectious Diseases, med fusion/Quest Diagnostics, Lewisville, Texas, USA.

Lara Poling (L)

AthoGen Testing, Carlsbad, California, USA.

Michael C Kurz (MC)

Department of Emergency Medicine, University of Alabama School of Medicine, Birmingham, Alabama, USA.

Ajit P Limaye (AP)

University of Washington, Seattle, Washington, USA.

Frank LoVecchio (F)

University of Arizona, Maricopa Medical Center, Phoenix, Arizona, USA.

Kristin Lowery (K)

AthoGen Testing, Carlsbad, California, USA.

Loren G Miller (LG)

Lundquist Institute at Harbor-UCLA Medical Center, Torrance, California, USA.

Gregory J Moran (GJ)

Department of Emergency Medicine, Olive View-UCLA Medical Center, Sylmar, California, USA.

J Scott Overcash (JS)

eStudySite, Sharp Chula Vista, San Diego, California, USA.

Amisha Parekh (A)

Department of Emergency Medicine, New York Methodist Hospital, New York, New York, USA.

W Frank Peacock (WF)

Baylor College of Medicine, Houston, Texas, USA.

Emanuel P Rivers (EP)

Department of Emergency Medicine and Surgery, Henry Ford Hospital, Detroit, Michigan, USA.
Department of Surgery, Henry Ford Hospital, Detroit, Michigan, USA.

Matthew Sims (M)

Beaumont Health, Royal Oak and Oakland University William Beaumont School of Medicine, Rochester, Michigan, USA.

Amy M Stubbs (AM)

Department of Emergency Medicine, Truman Medical Center, University of Missouri-Kansas City, Kansas City, Missouri, USA.

Martin Sundqvist (M)

Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Måns Ullberg (M)

Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.
Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.

Karen C Carroll (KC)

Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques microbiologiques › Hémoculture › Performance of PCR/Electrospray...
questionsmedicales.fr › Régions géographiques › Europe › Performance of PCR/Electrospray...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Performance of PCR/Electrospray...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Performance of PCR/Electrospray...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Inflammation › Sepsie › Performance of PCR/Electrospray...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse ESI › Performance of PCR/Electrospray...