Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophages.
Animals
Cell Line
Inflammation
/ chemically induced
Interleukin-18
/ metabolism
Interleukin-1beta
/ metabolism
Lipopolysaccharides
/ toxicity
Macrophages
/ metabolism
Mice
NLR Family, Pyrin Domain-Containing 3 Protein
/ metabolism
Naphthoquinones
/ pharmacology
Nitric Oxide
/ metabolism
Reactive Oxygen Species
/ metabolism
IL-18
IL-1β
NLRP3 inflammasome
caspase-1
juglone
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
07 Jul 2020
07 Jul 2020
Historique:
received:
19
06
2020
revised:
02
07
2020
accepted:
05
07
2020
entrez:
11
7
2020
pubmed:
11
7
2020
medline:
26
2
2021
Statut:
epublish
Résumé
The NLRP3 (NACHT, LRR and PYD domains-containing protein 3) inflammasome has been implicated in a variety of diseases, including atherosclerosis, neurodegenerative diseases, and infectious diseases. Thus, inhibitors of NLRP3 inflammasome have emerged as promising approaches to treat inflammation-related diseases. The aim of this study was to explore the effects of juglone (5-hydroxyl-1,4-naphthoquinone) on NLRP3 inflammasome activation. The inhibitory effects of juglone on nitric oxide (NO) production were assessed in lipopolysaccharide (LPS)-stimulated J774.1 cells by Griess assay, while its effects on reactive oxygen species (ROS) and NLRP3 ATPase activity were assessed. The expression levels of NLRP3, caspase-1, and pro-inflammatory cytokines (IL-1β, IL-18) and cytotoxicity of juglone in J774.1 cells were also determined. Juglone was non-toxic in J774.1 cells when used at 10 μM (
Identifiants
pubmed: 32646056
pii: molecules25133104
doi: 10.3390/molecules25133104
pmc: PMC7412499
pii:
doi:
Substances chimiques
IL1B protein, mouse
0
Interleukin-18
0
Interleukin-1beta
0
Lipopolysaccharides
0
NLR Family, Pyrin Domain-Containing 3 Protein
0
Naphthoquinones
0
Nlrp3 protein, mouse
0
Reactive Oxygen Species
0
Nitric Oxide
31C4KY9ESH
juglone
W6Q80SK9L6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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