Identification of the initial nucleocapsid recognition element in the HIV-1 RNA packaging signal.

Base Sequence Binding Sites Genome, Viral HIV Infections / virology HIV-1 / physiology Nucleic Acid Conformation Nucleocapsid / metabolism Nucleocapsid Proteins / metabolism Protein Binding RNA, Viral Regulatory Sequences, Ribonucleic Acid Virus Assembly
HIV-1 RNA genome nucleocapsid packaging

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
28 07 2020
Historique:
pubmed: 11 7 2020
medline: 18 9 2020
entrez: 11 7 2020
Statut: ppublish

Résumé

Selective packaging of the HIV-1 genome during virus assembly is mediated by interactions between the dimeric 5'-leader of the unspliced viral RNA and the nucleocapsid (NC) domains of a small number of assembling viral Gag polyproteins. Here, we show that the dimeric 5'-leader contains more than two dozen NC binding sites with affinities ranging from 40 nM to 1.4 μM, and that all high-affinity sites (

Identifiants

DOI: 10.1073/pnas.2008519117 PMID: 32647061 PMC: PMC7395439
pubmed: 32647061
pii: 2008519117
doi: 10.1073/pnas.2008519117
pmc: PMC7395439
doi:

Substances chimiques

Nucleocapsid Proteins 0
RNA, Viral 0
Regulatory Sequences, Ribonucleic Acid 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

17737-17746

Subventions

Organisme : NIAID NIH HHS
ID : U54 AI150470
Pays : United States
Organisme : NIGMS NIH HHS
ID : R25 GM055036
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NIGMS NIH HHS
ID : TL4 GM118989
Pays : United States
Organisme : NIGMS NIH HHS
ID : RL5 GM118987
Pays : United States
Organisme : NIGMS NIH HHS
ID : T34 GM008663
Pays : United States
Organisme : NIMHD NIH HHS
ID : TL4 MD009632
Pays : United States
Organisme : NIGMS NIH HHS
ID : UL1 GM118988
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL120826
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI150498
Pays : United States
Organisme : NIGMS NIH HHS
ID : T34 GM136497
Pays : United States

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2020 the Author(s). Published by PNAS.

Déclaration de conflit d'intérêts

The authors declare no competing interest.

Références

Curr HIV Res. 2006 Jan;4(1):43-55
pubmed: 16454710
J Virol. 1998 Jul;72(7):5886-96
pubmed: 9621050
J Virol. 1993 Jul;67(7):3997-4005
pubmed: 8510213
J Mol Biol. 2012 Mar 30;417(3):224-39
pubmed: 22306406
J Mol Biol. 2000 Aug 11;301(2):491-511
pubmed: 10926523
J Mol Biol. 2005 Jun 24;349(5):976-88
pubmed: 15907938
J Mol Biol. 1990 Dec 5;216(3):689-99
pubmed: 2124274
RNA. 1999 Sep;5(9):1268-72
pubmed: 10496227
J Mol Biol. 2007 Jan 12;365(2):453-67
pubmed: 17070546
J Virol. 1999 Jan;73(1):81-91
pubmed: 9847310
Proc Natl Acad Sci U S A. 1990 Feb;87(4):1556-60
pubmed: 2304918
J Virol. 2008 Mar;82(5):2376-84
pubmed: 18094172
Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13535-40
pubmed: 19628694
Adv Pharmacol. 2007;55:1-32
pubmed: 17586311
Nature. 2004 Sep 30;431(7008):586-90
pubmed: 15457265
Biochemistry. 2013 Dec 23;52(51):9269-74
pubmed: 24358934
Microbiol Mol Biol Rev. 2009 Sep;73(3):451-80, Table of Contents
pubmed: 19721086
Proc Natl Acad Sci U S A. 2009 Nov 10;106(45):19114-9
pubmed: 19861549
J Virol. 1990 May;64(5):1920-6
pubmed: 2109098
Science. 1990 Sep 14;249(4974):1281-5
pubmed: 2205002
Science. 2011 Oct 14;334(6053):242-5
pubmed: 21998393
Science. 2015 May 22;348(6237):917-21
pubmed: 25999508
Science. 2020 Apr 24;368(6489):413-417
pubmed: 32327595
Structure. 2018 Mar 6;26(3):490-498.e3
pubmed: 29398526
J Virol. 2007 Jul;81(14):7620-8
pubmed: 17494080
J Biomol NMR. 2016 Aug;65(3-4):205-216
pubmed: 27457481
J Virol. 2020 Feb 14;94(5):
pubmed: 31801870
Science. 1990 Nov 30;250(4985):1227-33
pubmed: 1700865
Methods Mol Biol. 2004;278:313-52
pubmed: 15318002
J Mol Biol. 2011 Jul 22;410(4):609-33
pubmed: 21762803
Nat Rev Microbiol. 2005 Aug;3(8):643-55
pubmed: 16064056
Semin Cell Dev Biol. 2019 Feb;86:129-139
pubmed: 29580971
Curr Top Microbiol Immunol. 1996;214:177-218
pubmed: 8791728
Annu Rev Virol. 2018 Sep 29;5(1):165-183
pubmed: 30048218
Proc Natl Acad Sci U S A. 2016 Nov 22;113(47):13378-13383
pubmed: 27834211
Sci Rep. 2015 Dec 03;5:17680
pubmed: 26631448
Science. 1998 Jan 16;279(5349):384-8
pubmed: 9430589
Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7297-301
pubmed: 7638184
J Virol. 1990 Jul;64(7):3207-11
pubmed: 2191147
J Virol. 2005 Nov;79(21):13528-37
pubmed: 16227273
Virus Res. 2013 Feb;171(2):278-86
pubmed: 22846919
PLoS Pathog. 2010 Oct 07;6(10):e1001007
pubmed: 20949075
Nucleic Acids Res. 2003 Feb 1;31(3):850-9
pubmed: 12560480
J Virol. 1990 Oct;64(10):5230-4
pubmed: 2204725
Biochemistry. 2008 Dec 9;47(49):13064-73
pubmed: 19006324
J Virol. 1996 May;70(5):2963-73
pubmed: 8627772
Nat Rev Microbiol. 2004 Jun;2(6):461-72
pubmed: 15152202
J Virol. 2003 Jan;77(1):84-96
pubmed: 12477813
J Mol Biol. 2011 Jul 22;410(4):501-11
pubmed: 21762796
Cold Spring Harb Perspect Med. 2012 Jul;2(7):a006924
pubmed: 22762019
Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):13033-13038
pubmed: 27791166
Prog Nucleic Acid Res Mol Biol. 1996;54:1-34
pubmed: 8768071
J Virol. 2004 Oct;78(19):10814-9
pubmed: 15367648
J Mol Biol. 2000 Apr 7;297(4):877-93
pubmed: 10736224
J Mol Biol. 2001 Nov 23;314(2):217-32
pubmed: 11718556
Nucleic Acids Res. 2018 Aug 21;46(14):7366-7378
pubmed: 29846681
J Virol. 1989 Sep;63(9):4085-7
pubmed: 2760989
Proc Natl Acad Sci U S A. 1994 May 24;91(11):4945-9
pubmed: 8197162
Viruses. 2016 Sep 10;8(9):
pubmed: 27626439
J Virol. 1997 May;71(5):3407-14
pubmed: 9094610
Curr Opin Struct Biol. 2010 Feb;20(1):114-20
pubmed: 20060706
Nat Commun. 2014 Jul 02;5:4304
pubmed: 24986025
Retrovirology. 2018 Mar 14;15(1):25
pubmed: 29540207

Auteurs

Pengfei Ding (P)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.
Howard Hughes Medical Institute, University of Maryland, Baltimore County, Baltimore, MD 21250.
ORCID: 0000-0003-1824-724X

Siarhei Kharytonchyk (S)

Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109.

Alexis Waller (A)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.
ORCID: 0000-0002-1435-7911

Ugonna Mbaekwe (U)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.

Sapna Basappa (S)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.

Nansen Kuo (N)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.

Heather M Frank (HM)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.

Christina Quasney (C)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.

Aaron Kidane (A)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.

Canessa Swanson (C)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.
ORCID: 0000-0002-8005-7562

Verna Van (V)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.

Mitali Sarkar (M)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.

Emily Cannistraci (E)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.
ORCID: 0000-0003-1880-9185

Ridhi Chaudhary (R)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.
ORCID: 0000-0003-0387-6342

Hana Flores (H)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250.

Alice Telesnitsky (A)

Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109 ateles@umich.edu summers@hhmi.umbc.edu.
ORCID: 0000-0001-9878-5931

Michael F Summers (MF)

Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21250; ateles@umich.edu summers@hhmi.umbc.edu.
Howard Hughes Medical Institute, University of Maryland, Baltimore County, Baltimore, MD 21250.
ORCID: 0000-0003-4267-4380

Articles similaires

Complete genome sequences of two novel Ralstonia jumbo phages isolated from leaf litter compost.

Ryota Sasaki, Shuhei Miyashita, Hideki Takahashi
1.00
Genome, Viral Ralstonia Composting Solanum lycopersicum Bacteriophages

Membrane potential stimulates ADP import and ATP export by the mitochondrial ADP/ATP carrier due to its positively charged binding site.

Vasiliki Mavridou, Martin S King, Andre Bazzone et al.
1.00
Adenosine Triphosphate Adenosine Diphosphate Mitochondrial ADP, ATP Translocases Binding Sites Mitochondria

Conservation of the cooling agent binding pocket within the TRPM subfamily.

Kate Huffer, Matthew C S Denley, Elisabeth V Oskoui et al.
1.00
TRPM Cation Channels Animals Binding Sites Mice Pyrimidinones

Drug repurposing against fucosyltransferase-2 via docking, STD-NMR, and molecular dynamic simulation studies.

Muhammad Atif, Humaira Zafar, Atia-Tul- Wahab et al.
1.00
Fucosyltransferases Drug Repositioning Molecular Docking Simulation Molecular Dynamics Simulation Humans

Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence nucléotidique › Identification of the initial nucleocapsid...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › Identification of the initial nucleocapsid...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome viral › Identification of the initial nucleocapsid...
questionsmedicales.fr › Maladies du système immunitaire › Déficits immunitaires › Infections à VIH › Identification of the initial nucleocapsid...
questionsmedicales.fr › Virus › Virus à ARN › Retroviridae › Lentivirus › Lentivirus des primates › VIH (Virus de l'Immunodéficience Humaine) › VIH-1 (Virus de l'Immunodéficience Humaine de type 1) › Identification of the initial nucleocapsid...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › Identification of the initial nucleocapsid...
questionsmedicales.fr › Structures virales › Virion › Nucléocapside › Identification of the initial nucleocapsid...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines nucléocapside › Identification of the initial nucleocapsid...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Identification of the initial nucleocapsid...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN viral › Identification of the initial nucleocapsid...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Séquences d'acides nucléiques régulatrices › Séquences régulatrices de l'acide ribonucléique › Identification of the initial nucleocapsid...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › Assemblage viral › Identification of the initial nucleocapsid...