Noscapine protects the H9c2 cardiomyocytes of rats against oxygen-glucose deprivation/reperfusion injury.
Animals
Antitussive Agents
/ pharmacology
Apoptosis
/ drug effects
Cell Hypoxia
/ drug effects
Cell Line
Cell Survival
/ drug effects
Glucose
/ deficiency
Male
Myocardial Reperfusion Injury
/ metabolism
Myocytes, Cardiac
/ cytology
Nitric Oxide
/ metabolism
Noscapine
/ pharmacology
Oxygen
/ metabolism
Rats
Apoptosis
H9c2 cardiomyocyte
Nitric oxide
Noscapine
Oxygen–glucose deprivation
Sigma receptors
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
16
03
2020
accepted:
23
05
2020
pubmed:
11
7
2020
medline:
23
6
2021
entrez:
11
7
2020
Statut:
ppublish
Résumé
Noscapine is an antitumor alkaloid derived from Papaver somniferum plants. Our previous study has demonstrated that exposure of noscapine on primary murine fetal cortical neurons exposed to oxygen-glucose deprivation/reperfusion (OGD/R) has neuroprotective effects. In current study, the effects of noscapine on cardiomyocytes (H9c2 cells) damage caused by 120 minutes (min) of OGD/R were evaluated and we determined whether the addition of BD1047, sigma-one receptor antagonist, prevents the protective effects of noscapine in H9c2 cells through the production of nitric oxide (NO) and apoptosis. To initiate OGD, H9c2 cells was transferred to glucose-free DMEM, and placed in a humidified incubation chamber. Cell viability was assessed with noscapine (1-5 μM) in the presence or absence of BD1047, 24 hours (h) after OGD/R. Cell viability, NO production and apoptosis ratio were evaluated by the MTT assay, the Griess method and the quantitative real-time PCR. Noscapine considerably improved the survival of H9c2 cells compared to OGD/R. Also, noscapine was extremely capable of reducing the concentrations of NO and Bax/Bcl-2 ratio expression. While the BD1047 administration alone diminished cell viability and increased the Bax/Bcl-2 ratio and NO levels. The addition of noscapine in the presence of BD1047 did not increase the cell viability relative to noscapine alone. Noscapine exerted cardioprotective effects exposed to OGD/R-induced injury in H9c2 cells, at least partly via attenuation of NO production and Bax/Bcl-2 ratio, which indicates that the sigma-one receptor activation is involved in the protection by noscapine of H9c2 cells injured by OGD/R.
Identifiants
pubmed: 32648076
doi: 10.1007/s11033-020-05549-6
pii: 10.1007/s11033-020-05549-6
doi:
Substances chimiques
Antitussive Agents
0
Nitric Oxide
31C4KY9ESH
Noscapine
8V32U4AOQU
Glucose
IY9XDZ35W2
Oxygen
S88TT14065
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5711-5719Subventions
Organisme : Iran University of Medical Sciences
ID : IR.IUMS.REC1393.25042
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