[FeFe]-hydrogenase maturation: H-cluster assembly intermediates tracked by electron paramagnetic resonance, infrared, and X-ray absorption spectroscopy.


Journal

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
ISSN: 1432-1327
Titre abrégé: J Biol Inorg Chem
Pays: Germany
ID NLM: 9616326

Informations de publication

Date de publication:
08 2020
Historique:
received: 21 04 2020
accepted: 09 06 2020
pubmed: 15 7 2020
medline: 21 5 2021
entrez: 15 7 2020
Statut: ppublish

Résumé

[FeFe]-hydrogenase enzymes employ a unique organometallic cofactor for efficient and reversible hydrogen conversion. This so-called H-cluster consists of a [4Fe-4S] cubane cysteine linked to a diiron complex coordinated by carbon monoxide and cyanide ligands and an azadithiolate ligand (adt = NH(CH

Identifiants

pubmed: 32661785
doi: 10.1007/s00775-020-01799-8
pii: 10.1007/s00775-020-01799-8
pmc: PMC7399679
doi:

Substances chimiques

Iron-Sulfur Proteins 0
iron hydrogenase EC 1.12.-
Hydrogenase EC 1.12.7.2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

777-788

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Auteurs

Brigitta Németh (B)

Department of Chemistry, Ångström Laboratory, Molecular Biomimetics, Uppsala University, 75120, Uppsala, Sweden.
Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, 59717, USA.

Moritz Senger (M)

Physics Department, Molecular Biophysics, Freie Universität Berlin, 14195, Berlin, Germany.
Department of Chemistry, Ångström Laboratory, Physical Chemistry, Uppsala University, 75120, Uppsala, Sweden.

Holly J Redman (HJ)

Department of Chemistry, Ångström Laboratory, Molecular Biomimetics, Uppsala University, 75120, Uppsala, Sweden.

Pierre Ceccaldi (P)

Department of Chemistry, Ångström Laboratory, Molecular Biomimetics, Uppsala University, 75120, Uppsala, Sweden.

Joan Broderick (J)

Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, 59717, USA.

Ann Magnuson (A)

Department of Chemistry, Ångström Laboratory, Molecular Biomimetics, Uppsala University, 75120, Uppsala, Sweden.

Sven T Stripp (ST)

Physics Department, Molecular Biophysics, Freie Universität Berlin, 14195, Berlin, Germany.

Michael Haumann (M)

Physics Department, Biophysics of Metalloenzymes, Freie Universität Berlin, 14195, Berlin, Germany.

Gustav Berggren (G)

Department of Chemistry, Ångström Laboratory, Molecular Biomimetics, Uppsala University, 75120, Uppsala, Sweden. gustav.berggren@kemi.uu.se.

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Classifications MeSH