Cutting in-line with iron: ribosomal function and non-oxidative RNA cleavage.

Binding Sites Cations, Divalent / chemistry Iron / chemistry Magnesium / chemistry Metals / chemistry Oxidation-Reduction / drug effects RNA Cleavage / genetics Ribosomes / chemistry

Journal

Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011

Informations de publication

Date de publication:
04 09 2020
Historique:
accepted: 30 06 2020
revised: 23 06 2020
received: 21 11 2019
pubmed: 15 7 2020
medline: 21 10 2020
entrez: 15 7 2020
Statut: ppublish

Résumé

Divalent metal cations are essential to the structure and function of the ribosome. Previous characterizations of the ribosome performed under standard laboratory conditions have implicated Mg2+ as a primary mediator of ribosomal structure and function. Possible contributions of Fe2+ as a ribosomal cofactor have been largely overlooked, despite the ribosome's early evolution in a high Fe2+ environment, and the continued use of Fe2+ by obligate anaerobes inhabiting high Fe2+ niches. Here, we show that (i) Fe2+ cleaves RNA by in-line cleavage, a non-oxidative mechanism that has not previously been shown experimentally for this metal, (ii) the first-order in-line rate constant with respect to divalent cations is >200 times greater with Fe2+ than with Mg2+, (iii) functional ribosomes are associated with Fe2+ after purification from cells grown under low O2 and high Fe2+ and (iv) a small fraction of Fe2+ that is associated with the ribosome is not exchangeable with surrounding divalent cations, presumably because those ions are tightly coordinated by rRNA and deeply buried in the ribosome. In total, these results expand the ancient role of iron in biochemistry and highlight a possible new mechanism of iron toxicity.

Identifiants

DOI: 10.1093/nar/gkaa586 PMID: 32663277 PMC: PMC7470983
pubmed: 32663277
pii: 5871366
doi: 10.1093/nar/gkaa586
pmc: PMC7470983
doi:

Substances chimiques

Cations, Divalent 0
Metals 0
Iron E1UOL152H7
Magnesium I38ZP9992A

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

8663-8674

Subventions

Organisme : NIEHS NIH HHS
ID : R33 ES025661
Pays : United States

Informations de copyright

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

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Auteurs

Rebecca Guth-Metzler (R)

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
NASA Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Marcus S Bray (MS)

NASA Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332, USA.
School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Moran Frenkel-Pinter (M)

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
NASA Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Suttipong Suttapitugsakul (S)

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Claudia Montllor-Albalate (C)

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Jessica C Bowman (JC)

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
NASA Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Ronghu Wu (R)

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Amit R Reddi (AR)

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.

C Denise Okafor (CD)

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.

Jennifer B Glass (JB)

NASA Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.
School of Earth and Atmospheric Sciences, Georgia Institute of Technology, Atlanta, GA 30332, USA.

Loren Dean Williams (LD)

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
NASA Center for the Origin of Life, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA.

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Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › Cutting in-line with iron: ribosomal function...
questionsmedicales.fr › Produits chimiques inorganiques › Électrolytes › Ions › Cations › Cations divalents › Cutting in-line with iron: ribosomal function...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Fer › Cutting in-line with iron: ribosomal function...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux légers › Magnésium › Cutting in-line with iron: ribosomal function...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Cutting in-line with iron: ribosomal function...
questionsmedicales.fr › Métabolisme › Métabolisme énergétique › Oxydoréduction › Cutting in-line with iron: ribosomal function...
questionsmedicales.fr › Phénomènes génétiques › Clivage de l'ARN › Cutting in-line with iron: ribosomal function...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Ribosomes › Cutting in-line with iron: ribosomal function...