The golgin family exhibits a propensity to form condensates in living cells.
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
23
05
2020
revised:
07
07
2020
accepted:
07
07
2020
pubmed:
16
7
2020
medline:
13
5
2021
entrez:
16
7
2020
Statut:
ppublish
Résumé
The Golgi is surrounded by a ribosome-excluding matrix. Recently, we reported that the cis-Golgi-localized golgin GM130 can phase-separate to form dynamic, liquid-like condensates in vitro and in vivo. Here, we show that the overexpression of each of the remaining cis (golgin160, GMAP210)- and trans (golgin97, golgin245, GCC88, GCC185)-golgins results in novel protein condensates. Focused ion beam scanning electron microscopy (FIB-SEM) images of GM130 condensates reveal a complex internal organization with branching aqueous channels. Pairs of golgins overexpressed in the same cell form distinct juxtaposed condensates. These findings support the hypothesis that, in addition to their established roles as vesicle tethers, phase separation may be a common feature of the golgin family that contributes to Golgi organization.
Identifiants
pubmed: 32668013
doi: 10.1002/1873-3468.13884
pmc: PMC7589415
doi:
Substances chimiques
Autoantigens
0
Golgi Matrix Proteins
0
Golgi complex autoantigen, 97-kDa
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3086-3094Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK034989
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118084
Pays : United States
Organisme : NIH HHS
ID : S10 OD020142
Pays : United States
Informations de copyright
© 2020 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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