ATP-Driven Separation of Liquid Phase Condensates in Bacteria.
ParABS
liquid droplets
liquid phase condensation
phase separation
photo-activated localization microscopy
plasmid partition
single particle tracking
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
16 07 2020
16 07 2020
Historique:
received:
17
09
2019
revised:
08
04
2020
accepted:
22
06
2020
entrez:
18
7
2020
pubmed:
18
7
2020
medline:
1
9
2020
Statut:
ppublish
Résumé
Liquid-liquid phase-separated (LLPS) states are key to compartmentalizing components in the absence of membranes; however, it is unclear whether LLPS condensates are actively and specifically organized in the subcellular space and by which mechanisms. Here, we address this question by focusing on the ParABS DNA segregation system, composed of a centromeric-like sequence (parS), a DNA-binding protein (ParB), and a motor (ParA). We show that parS and ParB associate to form nanometer-sized, round condensates. ParB molecules diffuse rapidly within the nucleoid volume but display confined motions when trapped inside ParB condensates. Single ParB molecules are able to rapidly diffuse between different condensates, and nucleation is strongly favored by parS. Notably, the ParA motor is required to prevent the fusion of ParB condensates. These results describe a novel active mechanism that splits, segregates, and localizes non-canonical LLPS condensates in the subcellular space.
Identifiants
pubmed: 32679076
pii: S1097-2765(20)30436-6
doi: 10.1016/j.molcel.2020.06.034
pii:
doi:
Substances chimiques
DNA, Bacterial
0
Escherichia coli Proteins
0
ParA protein, E coli
0
Adenosine Triphosphate
8L70Q75FXE
DNA Primase
EC 2.7.7.-
dnaG protein, E coli
EC 3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
293-303.e4Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.