Distinct conformational states of SARS-CoV-2 spike protein.
Angiotensin-Converting Enzyme 2
Cryoelectron Microscopy
HEK293 Cells
Host-Pathogen Interactions
/ immunology
Humans
Peptidyl-Dipeptidase A
/ chemistry
Protein Domains
Protein Multimerization
Protein Structure, Secondary
Receptors, Virus
/ chemistry
Spike Glycoprotein, Coronavirus
/ chemistry
Virus Internalization
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
25 09 2020
25 09 2020
Historique:
received:
20
06
2020
accepted:
14
07
2020
pubmed:
23
7
2020
medline:
22
10
2020
entrez:
23
7
2020
Statut:
ppublish
Résumé
Intervention strategies are urgently needed to control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The trimeric viral spike (S) protein catalyzes fusion between viral and target cell membranes to initiate infection. Here, we report two cryo-electron microscopy structures derived from a preparation of the full-length S protein, representing its prefusion (2.9-angstrom resolution) and postfusion (3.0-angstrom resolution) conformations, respectively. The spontaneous transition to the postfusion state is independent of target cells. The prefusion trimer has three receptor-binding domains clamped down by a segment adjacent to the fusion peptide. The postfusion structure is strategically decorated by N-linked glycans, suggesting possible protective roles against host immune responses and harsh external conditions. These findings advance our understanding of SARS-CoV-2 entry and may guide the development of vaccines and therapeutics.
Identifiants
pubmed: 32694201
pii: science.abd4251
doi: 10.1126/science.abd4251
pmc: PMC7464562
doi:
Substances chimiques
Receptors, Virus
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Peptidyl-Dipeptidase A
EC 3.4.15.1
ACE2 protein, human
EC 3.4.17.23
Angiotensin-Converting Enzyme 2
EC 3.4.17.23
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1586-1592Commentaires et corrections
Type : UpdateOf
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
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