Role of Nitric Oxide in the Change of 5-Hydroxytryptamine Synthesis in the Intestine by a Consecutive Administration of Methotrexate to Rats.
Animals
Drug Administration Schedule
Enzyme Inhibitors
/ administration & dosage
Injections, Intraperitoneal
Intestinal Diseases
/ chemically induced
Intestine, Small
/ drug effects
Male
Methotrexate
/ administration & dosage
NG-Nitroarginine Methyl Ester
/ administration & dosage
Nitric Oxide
/ metabolism
Nitric Oxide Synthase
/ antagonists & inhibitors
RNA, Messenger
/ metabolism
Rats
Rats, Wistar
Serotonin
/ biosynthesis
Substance P
/ drug effects
Tachykinins
/ drug effects
Tryptophan Hydroxylase
/ drug effects
5-Hydroxytryptamine
Methotrexate
Small intestine
Substance P
Tryptophan hydroxylase
Journal
Pharmacology
ISSN: 1423-0313
Titre abrégé: Pharmacology
Pays: Switzerland
ID NLM: 0152016
Informations de publication
Date de publication:
2020
2020
Historique:
received:
27
04
2020
accepted:
25
05
2020
pubmed:
23
7
2020
medline:
18
3
2021
entrez:
23
7
2020
Statut:
ppublish
Résumé
This study aimed to investigate whether the consecutive administration of methotrexate affects 5-hydroxytryptamine (5-HT) synthesis in the rat small intestine. Rats received methotrexate at a dose of 12.5 mg/kg intraperitoneally on 4 consecutive days. NG-nitro-L-arginine methyl ester (L-NAME) was given subcutaneously to inhibit nitric oxide (NO) synthase. Methotrexate moderately altered 5-HT synthesis, whereas the combined administration of methotrexate and L-NAME significantly changed 5-HT synthesis in the rat ileal tissue. These results suggest that endogenous NO has an antagonistic role in the induction of 5-HT synthesis in rats following the consecutive administration of methotrexate.
Identifiants
pubmed: 32694256
pii: 000508973
doi: 10.1159/000508973
doi:
Substances chimiques
Enzyme Inhibitors
0
RNA, Messenger
0
Tachykinins
0
Nitric Oxide
31C4KY9ESH
Serotonin
333DO1RDJY
Substance P
33507-63-0
Nitric Oxide Synthase
EC 1.14.13.39
Tryptophan Hydroxylase
EC 1.14.16.4
NG-Nitroarginine Methyl Ester
V55S2QJN2X
Methotrexate
YL5FZ2Y5U1
Types de publication
News
Langues
eng
Sous-ensembles de citation
IM
Pagination
723-728Informations de copyright
© 2020 S. Karger AG, Basel.