The closed eye harbours a unique microbiome in dry eye disease.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 07 2020
21 07 2020
Historique:
received:
31
12
2019
accepted:
23
04
2020
entrez:
23
7
2020
pubmed:
23
7
2020
medline:
18
12
2020
Statut:
epublish
Résumé
Dry eye affects millions of individuals. In experimental models, dry eye disease is associated with T helper cell 17-mediated inflammation of the ocular surface that may cause persistent damage to the corneal epithelium. However, the initiating and perpetuating factors associated with chronic inflammation of the ocular surface remain unclear. The ocular microbiota alters ocular surface inflammation and may influence dry eye disease development and progression. Here, we collected serial samples of tears on awakening from sleep, closed eye tears, during a randomized clinical trial of a non-pharmaceutical dry eye therapy and used 16S rRNA metabarcoding to characterize the microbiome. We show the closed dry eye microbiome is distinct from the healthy closed eye microbiome, and that the microbiome remains distinct despite daily saline eye wash upon awakening. The ocular microbiome was described only recently, and this report implicates a distinct microbiome in ocular disease development. Our findings suggest an interplay between microbial commensals and inflammation on the ocular surface. This information may inform future studies of the pathophysiological mechanisms of dry eye disease.
Identifiants
pubmed: 32694705
doi: 10.1038/s41598-020-68952-w
pii: 10.1038/s41598-020-68952-w
pmc: PMC7374690
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12035Subventions
Organisme : NIAID NIH HHS
ID : P30 AI027767
Pays : United States
Organisme : American Heart Association-American Stroke Association
ID : 17SDG32720009
Pays : United States
Organisme : NIH HHS
ID : K08HL141652
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR000165
Pays : United States
Organisme : NHLBI NIH HHS
ID : K08 HL141652
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL102371
Pays : United States
Organisme : NIH HHS
ID : R01HL102371
Pays : United States
Organisme : NIAMS NIH HHS
ID : P30 AR050948
Pays : United States
Organisme : CSRD VA
ID : I01 CX001969
Pays : United States
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