SARS-CoV-2 diagnostics in the virology laboratory of a University Hospital in Rome during the lockdown period.
Adolescent
Adult
Aged
Aged, 80 and over
Bronchoalveolar Lavage Fluid
/ virology
COVID-19
/ diagnosis
COVID-19 Testing
Child
Child, Preschool
Feces
/ virology
Female
Hospitals, University
Humans
Infant
Infant, Newborn
Laboratories
Male
Middle Aged
Nasopharynx
/ virology
Pandemics
Pleural Effusion
/ virology
Retrospective Studies
Reverse Transcriptase Polymerase Chain Reaction
Rome
/ epidemiology
SARS-CoV-2
/ genetics
Severity of Illness Index
COVID-19
SARS-CoV-2
epidemiology
laboratory diagnostics
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
28
06
2020
revised:
14
07
2020
accepted:
15
07
2020
pubmed:
23
7
2020
medline:
15
1
2021
entrez:
23
7
2020
Statut:
ppublish
Résumé
Italy was one of the most affected nations by coronavirus disease 2019 outside China. The infections, initially limited to Northern Italy, spread to all other Italian regions. This study aims to provide a snapshot of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) epidemiology based on a single-center laboratory experience in Rome. The study retrospectively included 6565 subjects tested for SARS-CoV-2 at the Laboratory of Virology of Sapienza University Hospital in Rome from 6 March to 4 May. A total of 9995 clinical specimens were analyzed, including nasopharyngeal swabs, bronchoalveolar lavage fluids, gargle lavages, stools, pleural fluids, and cerebrospinal fluids. Positivity to SARS-CoV-2 was detected in 8% (527/6565) of individuals, increased with age, and was higher in male patients (P < .001). The number of new confirmed cases reached a peak on 18 March and then decreased. The virus was detected in respiratory samples, in stool and in pleural fluids, while none of gargle lavage or cerebrospinal fluid samples gave a positive result. This analysis allowed to gather comprehensive information on SARS-CoV-2 epidemiology in our area, highlighting positivity variations over time and in different sex and age group and the need for a continuous surveillance of the infection, mostly because the pandemic evolution remains unknown.
Identifiants
pubmed: 32697357
doi: 10.1002/jmv.26332
pmc: PMC7405396
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
886-891Informations de copyright
© 2020 Wiley Periodicals LLC.
Références
J Clin Microbiol. 2020 Apr 23;58(5):
pubmed: 32102856
Metabolism. 2020 Jul;108:154252
pubmed: 32353355
J Korean Med Sci. 2020 Feb 10;35(5):e61
pubmed: 32030925
J Immunol. 2017 May 15;198(10):4046-4053
pubmed: 28373583
Nature. 2020 Feb 25;:
pubmed: 33623145
Viruses. 2020 Jan 24;12(2):
pubmed: 31991541
Int J Environ Res Public Health. 2020 Apr 27;17(9):
pubmed: 32349259
J Am Acad Dermatol. 2020 Jul;83(1):308-309
pubmed: 32283245
Diagn Microbiol Infect Dis. 2020 Jan;96(1):114893
pubmed: 31677959
Lancet Infect Dis. 2020 Jun;20(6):697-706
pubmed: 32224310
J Infect. 2020 Aug;81(2):e102-e103
pubmed: 32425274
Emerg Infect Dis. 2020 Aug;26(8):1930-1933
pubmed: 32330112
J Clin Virol. 2020 Jun;127:104378
pubmed: 32353762
JAMA. 2020 Apr 28;323(16):1574-1581
pubmed: 32250385
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
N Engl J Med. 2020 Apr 30;382(18):1708-1720
pubmed: 32109013
Euro Surveill. 2020 May;25(19):
pubmed: 32431289
J Infect. 2020 Jul;81(1):147-178
pubmed: 32209381
Sci China Life Sci. 2020 May;63(5):706-711
pubmed: 32146694
JAMA Netw Open. 2020 May 1;3(5):e2010182
pubmed: 32459353
Cytokine Growth Factor Rev. 2020 Jun;53:33-37
pubmed: 32389499
J Med Virol. 2021 Feb;93(2):886-891
pubmed: 32697357
China CDC Wkly. 2020 Jan 24;2(4):61-62
pubmed: 34594763
Ann Ist Super Sanita. 2016 Apr-Jun;52(2):198-204
pubmed: 27364394
JAMA. 2020 May 12;323(18):1843-1844
pubmed: 32159775
J Biol Regul Homeost Agents. 2020 Apr 07;34(2):339-343
pubmed: 32253888
Acta Biomed. 2020 Apr 10;91(3-S):175-179
pubmed: 32275287