Metabolic Maturation Media Improve Physiological Function of Human iPSC-Derived Cardiomyocytes.


Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
21 07 2020
Historique:
received: 25 03 2020
revised: 15 05 2020
accepted: 26 06 2020
entrez: 23 7 2020
pubmed: 23 7 2020
medline: 29 4 2021
Statut: ppublish

Résumé

Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have enormous potential for the study of human cardiac disorders. However, their physiological immaturity severely limits their utility as a model system and their adoption for drug discovery. Here, we describe maturation media designed to provide oxidative substrates adapted to the metabolic needs of human iPSC (hiPSC)-CMs. Compared with conventionally cultured hiPSC-CMs, metabolically matured hiPSC-CMs contract with greater force and show an increased reliance on cardiac sodium (Na

Identifiants

pubmed: 32697997
pii: S2211-1247(20)30906-2
doi: 10.1016/j.celrep.2020.107925
pmc: PMC7437654
mid: NIHMS1613944
pii:
doi:

Substances chimiques

Culture Media 0
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

107925

Subventions

Organisme : NHLBI NIH HHS
ID : R01 HL138539
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL132225
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL130840
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL141084
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL113601
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL128072
Pays : United States
Organisme : NHLBI NIH HHS
ID : R21 HL141019
Pays : United States

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Interests The authors declare no competing interests. A patent application related to this work has been submitted.

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Auteurs

Dries A M Feyen (DAM)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA; Department of Cardiology, University Medical Center Utrecht, Utrecht, the Netherlands.

Wesley L McKeithan (WL)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA; Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, CA, USA; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.

Arne A N Bruyneel (AAN)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Sean Spiering (S)

Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, CA, USA; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.

Larissa Hörmann (L)

Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Bärbel Ulmer (B)

Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Hui Zhang (H)

Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.

Francesca Briganti (F)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Michaela Schweizer (M)

Electron Microscopy Unit, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Bence Hegyi (B)

Department of Pharmacology, University of California, Davis, Davis, CA, USA.

Zhandi Liao (Z)

Department of Pharmacology, University of California, Davis, Davis, CA, USA.

Risto-Pekka Pölönen (RP)

Department of Pharmacology, University of California, Davis, Davis, CA, USA.

Kenneth S Ginsburg (KS)

Department of Pharmacology, University of California, Davis, Davis, CA, USA.

Chi Keung Lam (CK)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Ricardo Serrano (R)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Christine Wahlquist (C)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA; Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, CA, USA; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.

Alexander Kreymerman (A)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Michelle Vu (M)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Prashila L Amatya (PL)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Charlotta S Behrens (CS)

Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Sara Ranjbarvaziri (S)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Renee G C Maas (RGC)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA; Department of Cardiology, University Medical Center Utrecht, Utrecht, the Netherlands.

Matthew Greenhaw (M)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Daniel Bernstein (D)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Joseph C Wu (JC)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA.

Donald M Bers (DM)

Department of Pharmacology, University of California, Davis, Davis, CA, USA.

Thomas Eschenhagen (T)

Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Christian M Metallo (CM)

Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.

Mark Mercola (M)

Cardiovascular Institute and Department of Medicine, Stanford University, Stanford, CA 94305, USA; Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, CA, USA; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA. Electronic address: mmercola@stanford.edu.

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Classifications MeSH