Structural mechanism for gating of a eukaryotic mechanosensitive channel of small conductance.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
23 07 2020
23 07 2020
Historique:
received:
22
03
2020
accepted:
07
07
2020
entrez:
25
7
2020
pubmed:
25
7
2020
medline:
9
9
2020
Statut:
epublish
Résumé
Mechanosensitive ion channels transduce physical force into electrochemical signaling that underlies an array of fundamental physiological processes, including hearing, touch, proprioception, osmoregulation, and morphogenesis. The mechanosensitive channels of small conductance (MscS) constitute a remarkably diverse superfamily of channels critical for management of osmotic pressure. Here, we present cryo-electron microscopy structures of a MscS homolog from Arabidopsis thaliana, MSL1, presumably in both the closed and open states. The heptameric MSL1 channel contains an unusual bowl-shaped transmembrane region, which is reminiscent of the evolutionarily and architecturally unrelated mechanosensitive Piezo channels. Upon channel opening, the curved transmembrane domain of MSL1 flattens and expands. Our structures, in combination with functional analyses, delineate a structural mechanism by which mechanosensitive channels open under increased membrane tension. Further, the shared structural feature between unrelated channels suggests the possibility of a unified mechanical gating mechanism stemming from membrane deformation induced by a non-planar transmembrane domain.
Identifiants
pubmed: 32704140
doi: 10.1038/s41467-020-17538-1
pii: 10.1038/s41467-020-17538-1
pmc: PMC7378837
doi:
Substances chimiques
AT4G00290 protein, Arabidopsis
0
Arabidopsis Proteins
0
Escherichia coli Proteins
0
Ion Channels
0
MscS protein, E coli
0
Mutant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3690Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
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