An ATP-responsive metabolic cassette comprised of inositol tris/tetrakisphosphate kinase 1 (ITPK1) and inositol pentakisphosphate 2-kinase (IPK1) buffers diphosphosphoinositol phosphate levels.
Adenosine Triphosphate
/ metabolism
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
/ methods
Inositol Phosphates
/ analysis
Mesylates
/ chemistry
Mutation
Phosphorus Radioisotopes
Phosphorylation
Phosphotransferases (Alcohol Group Acceptor)
/ chemistry
Substrate Specificity
IPK1
ITPK1
diphosphoinositol phosphates
energy charge
phosphate homeostasis
Journal
The Biochemical journal
ISSN: 1470-8728
Titre abrégé: Biochem J
Pays: England
ID NLM: 2984726R
Informations de publication
Date de publication:
31 07 2020
31 07 2020
Historique:
received:
21
05
2020
revised:
29
06
2020
accepted:
06
07
2020
entrez:
25
7
2020
pubmed:
25
7
2020
medline:
13
2
2021
Statut:
ppublish
Résumé
Inositol polyphosphates are ubiquitous molecular signals in metazoans, as are their pyrophosphorylated derivatives that bear a so-called 'high-energy' phosphoanhydride bond. A structural rationale is provided for the ability of Arabidopsis inositol tris/tetrakisphosphate kinase 1 to discriminate between symmetric and enantiomeric substrates in the production of diverse symmetric and asymmetric myo-inositol phosphate and diphospho-myo-inositol phosphate (inositol pyrophosphate) products. Simple tools are applied to chromatographic resolution and detection of known and novel diphosphoinositol phosphates without resort to radiolabeling approaches. It is shown that inositol tris/tetrakisphosphate kinase 1 and inositol pentakisphosphate 2-kinase comprise a reversible metabolic cassette converting Ins(3,4,5,6)P4 into 5-InsP7 and back in a nucleotide-dependent manner. Thus, inositol tris/tetrakisphosphate kinase 1 is a nexus of bioenergetics status and inositol polyphosphate/diphosphoinositol phosphate metabolism. As such, it commands a role in plants that evolution has assigned to a different class of enzyme in mammalian cells. The findings and the methods described will enable a full appraisal of the role of diphosphoinositol phosphates in plants and particularly the relative contribution of reversible inositol phosphate hydroxykinase and inositol phosphate phosphokinase activities to plant physiology.
Identifiants
pubmed: 32706850
pii: 225707
doi: 10.1042/BCJ20200423
pmc: PMC7115839
mid: EMS86738
doi:
Substances chimiques
Arabidopsis Proteins
0
Inositol Phosphates
0
Mesylates
0
Phosphorus Radioisotopes
0
inositol heptakisphosphate
0
methanesulfonic acid
12EH9M7279
Phosphorus-32
690284A407
Adenosine Triphosphate
8L70Q75FXE
Phosphotransferases (Alcohol Group Acceptor)
EC 2.7.1.-
inositol 1,3,4,5,6-pentakisphosphate 2-kinase, Arabidopsis
EC 2.7.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2621-2638Subventions
Organisme : Wellcome Trust
ID : 101010
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M011216/1
Pays : United Kingdom
Informations de copyright
© 2020 The Author(s).
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