Protein-ligand binding with the coarse-grained Martini model.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
24 07 2020
Historique:
received: 29 08 2019
accepted: 29 06 2020
entrez: 26 7 2020
pubmed: 28 7 2020
medline: 9 9 2020
Statut: epublish

Résumé

The detailed understanding of the binding of small molecules to proteins is the key for the development of novel drugs or to increase the acceptance of substrates by enzymes. Nowadays, computer-aided design of protein-ligand binding is an important tool to accomplish this task. Current approaches typically rely on high-throughput docking essays or computationally expensive atomistic molecular dynamics simulations. Here, we present an approach to use the recently re-parametrized coarse-grained Martini model to perform unbiased millisecond sampling of protein-ligand interactions of small drug-like molecules. Remarkably, we achieve high accuracy without the need of any a priori knowledge of binding pockets or pathways. Our approach is applied to a range of systems from the well-characterized T4 lysozyme over members of the GPCR family and nuclear receptors to a variety of enzymes. The presented results open the way to high-throughput screening of ligand libraries or protein mutations using the coarse-grained Martini model.

Identifiants

pubmed: 32709852
doi: 10.1038/s41467-020-17437-5
pii: 10.1038/s41467-020-17437-5
pmc: PMC7382508
doi:

Substances chimiques

Ligands 0
Proteins 0
Muramidase EC 3.2.1.17

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3714

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Auteurs

Paulo C T Souza (PCT)

Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 7, 9747 AG, Groningen, Netherlands. paulocts@gmail.com.

Sebastian Thallmair (S)

Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 7, 9747 AG, Groningen, Netherlands.

Paolo Conflitti (P)

Faculty of Biomedical Sciences, Institute of Computational Science, Università della Svizzera italiana (USI), via G. Buffi 13, CH-6900, Lugano, Switzerland.

Carlos Ramírez-Palacios (C)

Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 7, 9747 AG, Groningen, Netherlands.

Riccardo Alessandri (R)

Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 7, 9747 AG, Groningen, Netherlands.

Stefano Raniolo (S)

Faculty of Biomedical Sciences, Institute of Computational Science, Università della Svizzera italiana (USI), via G. Buffi 13, CH-6900, Lugano, Switzerland.

Vittorio Limongelli (V)

Faculty of Biomedical Sciences, Institute of Computational Science, Università della Svizzera italiana (USI), via G. Buffi 13, CH-6900, Lugano, Switzerland. vittoriolimongelli@gmail.com.
Department of Pharmacy, University of Naples "Federico II", via D. Montesano 49, I-80131, Naples, Italy. vittoriolimongelli@gmail.com.

Siewert J Marrink (SJ)

Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 7, 9747 AG, Groningen, Netherlands. s.j.marrink@rug.nl.

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