The impaired quorum sensing response of Pseudomonas aeruginosa MexAB-OprM efflux pump overexpressing mutants is not due to non-physiological efflux of 3-oxo-C12-HSL.
4-Butyrolactone
/ analogs & derivatives
Anti-Bacterial Agents
/ metabolism
Bacterial Outer Membrane Proteins
/ genetics
Caprylates
/ metabolism
Drug Resistance, Bacterial
/ genetics
Homoserine
/ analogs & derivatives
Membrane Transport Proteins
/ genetics
Pseudomonas aeruginosa
/ genetics
Quinolones
/ metabolism
Quorum Sensing
/ genetics
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
02
04
2020
revised:
18
06
2020
accepted:
24
07
2020
pubmed:
28
7
2020
medline:
13
4
2021
entrez:
28
7
2020
Statut:
ppublish
Résumé
Multidrug (MDR) efflux pumps are ancient and conserved molecular machineries with relevant roles in different aspects of the bacterial physiology, besides antibiotic resistance. In the case of the environmental opportunistic pathogen Pseudomonas aeruginosa, it has been shown that overexpression of different efflux pumps is linked to the impairment of the quorum sensing (QS) response. Nevertheless, the causes of such impairment are different for each analysed efflux pump. Herein, we performed an in-depth analysis of the QS-mediated response of a P. aeruginosa antibiotic resistant mutant that overexpresses MexAB-OprM. Although previous work claimed that this efflux pump extrudes the QS signal 3-oxo-C12-HSL, we show otherwise. Our results evidence that the observed attenuation in the QS response when overexpressing this pump is related to an impaired production of alkyl quinolone QS signals, likely prompted by the reduced availability of one of their precursors, the octanoate. Together with previous studies, this indicates that, although the consequences of overexpressing efflux pumps are similar (impaired QS response), the underlying mechanisms are different. This 'apparent redundancy' of MDR efflux systems can be understood as a P. aeruginosa strategy to keep the robustness of the QS regulatory network and modulate its output in response to different signals.
Identifiants
pubmed: 32715566
doi: 10.1111/1462-2920.15177
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Outer Membrane Proteins
0
Caprylates
0
Membrane Transport Proteins
0
N-(3-oxododecanoyl)homoserine lactone
0
Quinolones
0
Homoserine
6KA95X0IVO
octanoic acid
OBL58JN025
4-Butyrolactone
OL659KIY4X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5167-5188Subventions
Organisme : Comunidad de Madrid
ID : B2017/BMD-3691
Organisme : Instituto de Salud Carlos III
ID : RD16/0016/0011
Organisme : Secretaría de Estado de Investigación, Desarrollo e Innovación
ID : BIO2017-83128-R
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R012415/1
Pays : United Kingdom
Informations de copyright
© 2020 Society for Applied Microbiology and John Wiley & Sons Ltd.
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