A Tissue-Engineered Human Psoriatic Skin Model to Investigate the Implication of cAMP in Psoriasis: Differential Impacts of Cholera Toxin and Isoproterenol on cAMP Levels of the Epidermis.
cholera toxin
cyclic adenosine monophosphate
isoproterenol
psoriasis
tissue engineering
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
23 Jul 2020
23 Jul 2020
Historique:
received:
11
06
2020
revised:
17
07
2020
accepted:
21
07
2020
entrez:
29
7
2020
pubmed:
29
7
2020
medline:
17
2
2021
Statut:
epublish
Résumé
Pathological and healthy skin models were reconstructed using similar culture conditions according to well-known tissue engineering protocols. For both models, cyclic nucleotide enhancers were used as additives to promote keratinocytes' proliferation. Cholera toxin (CT) and isoproterenol (ISO), a beta-adrenergic agonist, are the most common cAMP stimulators recommended for cell culture. The aim of this study was to evaluate the impact of either CT or ISO on the pathological characteristics of the dermatosis while producing a psoriatic skin model. Healthy and psoriatic skin substitutes were produced according to the self-assembly method of tissue engineering, using culture media supplemented with either CT (10
Identifiants
pubmed: 32717879
pii: ijms21155215
doi: 10.3390/ijms21155215
pmc: PMC7432929
pii:
doi:
Substances chimiques
FLG protein, human
0
Filaggrin Proteins
0
Cholera Toxin
9012-63-9
Cyclic AMP
E0399OZS9N
Adenylyl Cyclases
EC 4.6.1.1
adenylate cyclase 9
EC 4.6.1.1
Isoproterenol
L628TT009W
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : CIHR
ID : MOP-311262
Pays : Canada
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