Polymorphism in GSTM1 and GSTT1 genes influence DNA damage in personnel occupationally exposed to volatile anaesthetics (VA), from Peshawar, Pakistan.
Adult
Age Factors
Anesthesiology
/ statistics & numerical data
Anesthetics, Inhalation
/ adverse effects
Comet Assay
DNA Damage
/ genetics
Genetic Markers
/ genetics
Glutathione Transferase
/ genetics
Health Personnel
/ statistics & numerical data
Humans
Male
Occupational Exposure
/ adverse effects
Pakistan
/ epidemiology
Polymorphism, Genetic
/ genetics
Smoking
/ adverse effects
P450 cytochrome enzymes
biomonitoring
genotoxicity
international occupational health
toxicology
Journal
Occupational and environmental medicine
ISSN: 1470-7926
Titre abrégé: Occup Environ Med
Pays: England
ID NLM: 9422759
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
24
03
2020
revised:
15
06
2020
accepted:
17
06
2020
pubmed:
29
7
2020
medline:
28
11
2020
entrez:
29
7
2020
Statut:
ppublish
Résumé
The objective of this study was to assess the influence of antioxidant gene GSTM1 and GSTT1 on DNA damage in personnel occupationally exposed to volatile anaesthetics (VA). The study groups were composed of 50 exposed subjects (anaesthesia workers) and 49 controls. Blood samples were collected from both subjects. DNA damage was analysed through the comet assay technique. Biomarker genes GSTM1 and GSTT1 were inspected through PCR technique for polymorphism. The comet assay technique showed that the Total Comet Score (TCS) in exposed subjects was significantly higher (p=0.0001) than the control. Age and smoking had significant effects on TCS in the study groups (p<0.05). Duration of occupational exposure had significant positive correlation (r=0.755, p<0.001) with DNA damage. The null polymorphism in GSTM1 and GSTT1 gene showed a significant effect (p<0.001 and p<0.000) on the DNA damage. The polymorphism in GSTM1 and GSTT1 gene significantly damage DNA in personnel occupationally exposed to VA.
Identifiants
pubmed: 32719018
pii: oemed-2020-106561
doi: 10.1136/oemed-2020-106561
doi:
Substances chimiques
Anesthetics, Inhalation
0
Genetic Markers
0
glutathione S-transferase T1
EC 2.5.1.-
Glutathione Transferase
EC 2.5.1.18
glutathione S-transferase M1
EC 2.5.1.18
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
769-774Informations de copyright
© Author(s) (or their employer(s)) 2020. No commercial re-use. See rights and permissions. Published by BMJ.
Déclaration de conflit d'intérêts
Competing interests: None declared.