Rapid isolation of rare targets from large fluid volumes.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 07 2020
27 07 2020
Historique:
received:
01
05
2020
accepted:
26
06
2020
entrez:
29
7
2020
pubmed:
29
7
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Rapidly isolating rare targets from larger, clinically relevant fluid volumes remains an unresolved problem in biomedicine and diagnosis. Here, we describe how 3D particle sorting can enrich targets at ultralow concentrations over 100-fold within minutes not possible with conventional approaches. Current clinical devices based on biochemical extraction and microfluidic solutions typically require high concentrations and/or can only process sub-milliliter volumes in time. In a proof-of-concept application, we isolated bacteria from whole blood as demanded for rapid sepsis diagnosis where minimal numbers of bacteria need to be found in a 1-10 mL blood sample. After sample encapsulation in droplets and target enrichment with the 3D particle sorter within a few minutes, downstream analyses were able to identify bacteria and test for antibiotic susceptibility, information which is critical for successful treatment of bloodstream infections.
Identifiants
pubmed: 32719382
doi: 10.1038/s41598-020-69315-1
pii: 10.1038/s41598-020-69315-1
pmc: PMC7385493
doi:
Types de publication
Evaluation Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12458Subventions
Organisme : NIAID NIH HHS
ID : R01 AI117061
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103540
Pays : United States
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