Contribution of Streptococcus mutans to Helicobacter pylori colonisation in oral cavity and gastric tissue.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 07 2020
27 07 2020
Historique:
received:
02
04
2020
accepted:
07
07
2020
entrez:
29
7
2020
pubmed:
29
7
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Helicobacter pylori is presumed to infect gastric tissue via the oral cavity in childhood, whereas risk factors for H. pylori infection in the oral cavity are unknown. In this study, we analysed the effects of Streptococcus mutans, a major cariogenic bacterial species, on H. pylori colonisation in the oral cavity, as well as gastric tissue. Rats in the weaning period were infected with S. mutans in the oral cavity, then fed a caries-inducing diet to facilitate S. mutans colonisation. One month after S. mutans infection, rats were infected with H. pylori in the oral cavity; rats were then euthanised at 1 month after H. pylori infection. H. pylori was detected in the oral cavities of rats infected with both S. mutans and H. pylori, but not in rats infected with H. pylori alone. In addition, H. pylori colonisation in the gastric tissue and typical gastrointestinal damage were observed in rats infected with both S. mutans and H. pylori. When H. pylori was co-cultured with in vitro biofilm formed by S. mutans, a large number of H. pylori bacteria invaded the biofilm formed by S. mutans. Our results suggest that S. mutans is involved in the establishment of H. pylori infection.
Identifiants
pubmed: 32719470
doi: 10.1038/s41598-020-69368-2
pii: 10.1038/s41598-020-69368-2
pmc: PMC7385622
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12540Références
Fennerty, M. B. Helicobacter pylori. Arch. Intern. Med. 154, 721–727 (1994).
doi: 10.1001/archinte.1994.00420070021003
Hooi, J. K. Y. et al. Global prevalence of Helicobacter pylori infection: Systematic review and meta-analysis. Gastroenterology 153, 420–429 (2017).
doi: 10.1053/j.gastro.2017.04.022
Banatvala, N. et al. Migration and Helicobacter pylori seroprevalence: Bangladeshi migrants in the UK. J. Infect. 31, 133–135 (1995).
doi: 10.1016/S0163-4453(95)92135-4
Pounder, R. E. & Ng, D. The prevalence of Helicobacter pylori infection in different countries. Aliment. Pharmacol. Ther. 9(Suppl 2), 33–39 (1995).
pubmed: 8547526
Miyabayashi, H., Furihata, K., Shimizu, T., Ueno, I. & Akamatsu, T. Influence of oral Helicobacter pylori on the success of eradication therapy against gastric Helicobacter pylori. Helicobacter 5, 30–37 (2000).
doi: 10.1046/j.1523-5378.2000.00004.x
Wang, J. et al. Comparison of cytotoxin genotypes of Helicobacter pylori in stomach and saliva. Dig. Dis. Sci. 47, 1850–1856 (2002).
doi: 10.1023/A:1016417200611
Ogaya, Y., Nomura, R., Watanabe, Y. & Nakano, K. Detection of Helicobacter pylori DNA in inflamed dental pulp specimens from Japanese children and adolescents. J. Med. Microbiol. 64, 117–123 (2015).
doi: 10.1099/jmm.0.079491-0
Zou, Q. H. & Li, R. Q. Helicobacter pylori in the oral cavity and gastric mucosa: a meta-analysis. J. Oral. Pathol. Med. 40, 317–324 (2011).
doi: 10.1111/j.1600-0714.2011.01006.x
Hamada, S. & Slade, H. D. Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiol. Rev. 44, 331–384 (1980).
doi: 10.1128/MMBR.44.2.331-384.1980
Caufield, P. W., Cutter, G. R. & Dasanayake, A. P. Initial acquisition of mutans streptococci by infants: evidence for a discrete window of infectivity. J. Dent. Res. 72, 37–45 (1993).
doi: 10.1177/00220345930720010501
Keyes, P. H. Recent advances in dental caries research Bacteriology. Bacteriological findings and biological implications. Int. Dent. J. 12, 443–464 (1962).
Eckert, R. et al. Targeted killing of Streptococcus mutans by a pheromone-guided “smart” antimicrobial peptide. Antimicrob. Agents. Chemother. 50, 1480–1488 (2006).
doi: 10.1128/AAC.50.4.1480-1488.2006
Liu, Y. et al. Study on the relationship between Helicobacter pylori in the dental plaque and the occurrence of dental caries or oral hygiene index. Helicobacter 13, 256–260 (2008).
doi: 10.1111/j.1523-5378.2008.00602.x
Aksit Bıcak, D. et al. The investigation of Helicobacter pylori in the dental biofilm and saliva samples of children with dyspeptic complaints. BMC Oral. Health 17, 67 (2017).
doi: 10.1186/s12903-017-0361-x
Ooshima, T. et al. Comparison of the cariostatic effects between regimens to administer oolong tea polyphenols in SPF rats. Caries Res. 32, 75–80 (1998).
doi: 10.1159/000016433
Hoshino, T. et al. PCR detection and identification of oral streptococci in saliva samples using gtf genes. Diagn. Microbiol. Infect. Dis. 48, 195–199 (2004).
doi: 10.1016/j.diagmicrobio.2003.10.002
Nomura, R., Ogaya, Y., Matayoshi, S., Morita, Y. & Nakano, K. Molecular and clinical analyses of Helicobacter pylori colonization in inflamed dental pulp. BMC Oral Health 18, 64 (2018).
doi: 10.1186/s12903-018-0526-2
Yoshida, A. & Kuramitsu, H. K. Multiple Streptococcus mutans genes are involved in biofilm formation. Appl. Environ. Microbiol. 68, 6283–6291 (2002).
doi: 10.1128/AEM.68.12.6283-6291.2002
Ardin, A. C. et al. Identification and functional analysis of an ammonium transporter in Streptococcus mutans. PLoS ONE 9, e107569 (2014).
doi: 10.1371/journal.pone.0107569
Nomura, R., Morita, Y., Matayoshi, S. & Nakano, K. Inhibitory effect of surface pre-reacted glass-ionomer (S-PRG) elute against adhesion and colonization by Streptococcus mutans. Sci. Rep. 8, 5056 (2018).
doi: 10.1038/s41598-018-23354-x
Prasanthi, C. H., Prasanthi, N. L., Manikiran, S. S. & Rama-Rao, N. N. Focus on current trends in the treatment of Helicobacter pylori infection: an update. Inter. J. Pharm. Sci. Rev. Res. 1, 42–51 (2011).
Morales-Espinosa, R. et al. Helicobacter pylori in the oral cavity is associated with gastroesophageal disease. Oral Microbiol. Immunol. 24, 464–468 (2009).
doi: 10.1111/j.1399-302X.2009.00541.x
Silva, D. G. et al. Helicobacter pylori transiently in the mouth may participate in the transmission of infection. Mem. Inst. Oswaldo Cruz. 105, 657–660 (2010).
doi: 10.1590/S0074-02762010000500009
Yee, J. K. C. Are the view of Helicobacter pylori colonized in the oral cavity an illusion?. Exp. Mol. Med. 49, e397 (2017).
doi: 10.1038/emm.2017.225
Liu, P. et al. A cross-sectional survey of dental caries, oral hygiene, and Helicobacter pulori infection in adults. Asia Pac. J. Pub. Health 25, 49S-56S (2013).
doi: 10.1177/1010539513495555
Nomura, R., Matayoshi, S., Otsugu, M., Kitamura, T., Teramoto, N. & Nakano, K. Contribution of severe dental caries induced by Streptococcus mutans to the pathogenicity of infective endocarditis. Infect Immun 88, e00897-19 (2020).
doi: 10.1128/IAI.00897-19
Zhang, W., Deng, X., Zhou, X., Hao, Y. & Li, Y. Influence of Helicobacter pylori culture supernatant on the ecological balance of a dual-species oral biofilm. J. Appl. Oral Sci. 26, e20170113 (2018).
doi: 10.1590/1678-7757-2017-0113
Kuremoto, K. et al. Promotion of endodontic lesions in rats by a novel extraradicular biofilm model using obturation materials. Appl. Environ. Microbiol. 80, 3804–3810 (2014).
doi: 10.1128/AEM.00421-14
Smoot, D. T. How does Helicobacter pylori cause mucosal damage? Direct mechanisms. Gastroenterology 113, S31–S34 (1997).
doi: 10.1016/S0016-5085(97)80008-X
Gisbert, J. P. et al. Erosive duodenitis: prevalence of Helicobacter pylori infection and response to eradication therapy with omeprazole plus two antibiotics. Eur. J. Gastroenterol. Hepatol. 9, 957–962 (1997).
doi: 10.1097/00042737-199710000-00007
Wroblewski, L. E., Peek, R. M. Jr. & Wilson, K. T. Helicobacter pylori and gastric cancer: factors that modulate disease risk. Clin. Microbiol. Rev. 23, 713–739 (2010).
doi: 10.1128/CMR.00011-10
Ishihara, K. et al. Oral bacteria inhibit Helicobacter pylori growth. FEMS Microbiol. Lett. 152, 355–361 (1997).
doi: 10.1111/j.1574-6968.1997.tb10452.x
Yonezawa, H., Osaki, T. & Kamiya, S. Biofilm formation by Helicobacter pylori and its involvement for antibiotic resistance. Biomed. Res. Int. 2015, 914791 (2015).
doi: 10.1155/2015/914791
Kolenbrander, P. E. & London, J. Adhere today, here tomorrow: Oral bacterial adherence. J. Bacteriol. 175, 3247–3252 (1993).
doi: 10.1128/JB.175.11.3247-3252.1993
Nomura, R., Nakano, K. & Ooshima, T. Contribution of glucan-binding protein C of Streptococcus mutans to bacteremia occurrence. Arch. Oral. Biol. 49, 783–788 (2004).
doi: 10.1016/j.archoralbio.2004.04.001
Matsumoto-Nakano, M. et al. Inhibitory effect of Oenothera biennis (evening primrose) seed extract on Streptococcus mutans and S. mutans-induced dental caries in rats. Caries Res. 45, 56–63 (2011).
doi: 10.1159/000323376
Nolte, T. et al. Nonproliferative and proliferative lesions of the gastrointestinal tract, pancreas and salivary glands of the rat and mouse. J. Toxicol. Pathol. 29, 1S-125S (2016).
doi: 10.1293/tox.29.1S
Nakano, K., Tsuji, M., Nishimura, K., Nomura, R. & Ooshima, T. Contribution of cell surface protein antigen PAc of Streptococcus mutans to bacteremia. Microbes. Infect. 8, 114–121 (2006).
doi: 10.1016/j.micinf.2005.06.005