Social touch promotes interfemale communication via activation of parvocellular oxytocin neurons.


Journal

Nature neuroscience
ISSN: 1546-1726
Titre abrégé: Nat Neurosci
Pays: United States
ID NLM: 9809671

Informations de publication

Date de publication:
09 2020
Historique:
received: 10 01 2019
accepted: 18 06 2020
pubmed: 29 7 2020
medline: 1 12 2020
entrez: 29 7 2020
Statut: ppublish

Résumé

Oxytocin (OT) is a great facilitator of social life but, although its effects on socially relevant brain regions have been extensively studied, OT neuron activity during actual social interactions remains unexplored. Most OT neurons are magnocellular neurons, which simultaneously project to the pituitary and forebrain regions involved in social behaviors. In the present study, we show that a much smaller population of OT neurons, parvocellular neurons that do not project to the pituitary but synapse onto magnocellular neurons, is preferentially activated by somatosensory stimuli. This activation is transmitted to the larger population of magnocellular neurons, which consequently show coordinated increases in their activity during social interactions between virgin female rats. Selectively activating these parvocellular neurons promotes social motivation, whereas inhibiting them reduces social interactions. Thus, parvocellular OT neurons receive particular inputs to control social behavior by coordinating the responses of the much larger population of magnocellular OT neurons.

Identifiants

pubmed: 32719563
doi: 10.1038/s41593-020-0674-y
pii: 10.1038/s41593-020-0674-y
doi:

Substances chimiques

Oxytocin 50-56-6

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1125-1137

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S000224/1
Pays : United Kingdom
Organisme : NINDS NIH HHS
ID : R01 NS094640
Pays : United States

Commentaires et corrections

Type : CommentIn

Références

Lee, H.-J., Macbeth, A. H., Pagani, J. & Scott Young, W. 3rd. Oxytocin: the great facilitator of life. Prog. Neurobiol. 88, 127–151 (2010).
Jurek, B. & Neumann, I. D. The oxytocin receptor: from intracellular signaling to behavior. Physiol. Rev. 98, 1805–1908 (2018).
pubmed: 29897293 doi: 10.1152/physrev.00031.2017 pmcid: 29897293
Walum, H. & Young, L. J. The neural mechanisms and circuitry of the pair bond. Nat. Rev. Neurosci. 19, 643–654 (2018).
pubmed: 30301953 pmcid: 6283620 doi: 10.1038/s41583-018-0072-6
Russell, J. A., Leng, G. & Douglas, A. J. The magnocellular oxytocin system, the fount of maternity: adaptations in pregnancy. Front. Neuroendocrinol. 24, 27–61 (2003).
pubmed: 12609499 doi: 10.1016/S0091-3022(02)00104-8 pmcid: 12609499
Knobloch, H. S. et al. Evoked axonal oxytocin release in the central amygdala attenuates fear response. Neuron 73, 553–566 (2012).
pubmed: 22325206 doi: 10.1016/j.neuron.2011.11.030 pmcid: 22325206
Marlin, B. J. & Froemke, R. C. Oxytocin modulation of neural circuits for social behavior. Dev. Neurobiol. 77, 169–189 (2017).
pubmed: 27626613 doi: 10.1002/dneu.22452 pmcid: 27626613
Grinevich, V. & Stoop, R. Interplay between oxytocin and sensory systems in the orchestration of socio-emotional behaviors. Neuron 99, 887–904 (2018).
pubmed: 30189208 doi: 10.1016/j.neuron.2018.07.016 pmcid: 30189208
Dumais, K. M., Alonso, A. G., Immormino, M. A., Bredewold, R. & Veenema, A. H. Involvement of the oxytocin system in the bed nucleus of the stria terminalis in the sex-specific regulation of social recognition. Psychoneuroendocrinology 64, 79–88 (2016).
pubmed: 26630388 doi: 10.1016/j.psyneuen.2015.11.007 pmcid: 26630388
Dumais, K. M., Alonso, A. G., Bredewold, R. & Veenema, A. H. Role of the oxytocin system in amygdala subregions in the regulation of social interest in male and female rats. Neuroscience 330, 138–149 (2016).
pubmed: 27235738 pmcid: 4927343 doi: 10.1016/j.neuroscience.2016.05.036
Resendez, S. L. et al. Social stimuli induce activation of oxytocin neurons within the paraventricular nucleus of the hypothalamus to promote social behavior in male mice. J. Neurosci. 40, 2282–2295 (2020).
pubmed: 32024781 pmcid: 7083279 doi: 10.1523/JNEUROSCI.1515-18.2020
Bobrov, E., Wolfe, J., Rao, R. P. & Brecht, M. The representation of social facial touch in rat barrel cortex. Curr. Biol. 24, 109–115 (2014).
pubmed: 24361064 doi: 10.1016/j.cub.2013.11.049 pmcid: 24361064
Chen, P. & Hong, W. Neural circuit mechanisms of social behavior. Neuron 98, 16–30 (2018).
pubmed: 29621486 pmcid: 6028944 doi: 10.1016/j.neuron.2018.02.026
Eliava, M. et al. A new population of parvocellular oxytocin neurons controlling magnocellular neuron activity and inflammatory pain processing. Neuron 89, 1291–1304 (2016).
pubmed: 26948889 pmcid: 5679079 doi: 10.1016/j.neuron.2016.01.041
Lima, S. Q., Hromádka, T., Znamenskiy, P. & Zador, A. M. PINP: a new method of tagging neuronal populations for identification during in vivo electrophysiological recording. PLoS ONE 4, e6099 (2009).
pubmed: 19584920 pmcid: 2702752 doi: 10.1371/journal.pone.0006099
Leng, T., Leng, G. & MacGregor, D. J. Spike patterning in oxytocin neurons: capturing physiological behaviour with Hodgkin–Huxley and integrate-and-fire models. PLoS ONE 12, e0180368 (2017).
pubmed: 28683135 pmcid: 5500322 doi: 10.1371/journal.pone.0180368
Netser, S., Haskal, S., Magalnik, H. & Wagner, S. A novel system for tracking social preference dynamics in mice reveals sex- and strain-specific characteristics. Mol. Autism 8, 53 (2017).
pubmed: 29026510 pmcid: 5627457 doi: 10.1186/s13229-017-0169-1
Maícas-Royo, J., Leng, G. & MacGregor, D. J. A predictive, quantitative model of spiking activity and stimulus-secretion coupling in oxytocin neurons. Endocrinology 159, 1433–1452 (2018).
pubmed: 29342276 pmcid: 5934744 doi: 10.1210/en.2017-03068
Portfors, C. V. Types and functions of ultrasonic vocalizations in laboratory rats and mice. J. Am. Assoc. Lab. Anim. Sci. 46, 28–34 (2007).
pubmed: 17203913 pmcid: 17203913
Lenschow, C. et al. Sexually monomorphic maps and dimorphic responses in rat genital cortex. Curr. Biol. 26, 106–113 (2016).
pubmed: 26725197 doi: 10.1016/j.cub.2015.11.041 pmcid: 26725197
Althammer, F. & Grinevich, V. Diversity of oxytocin neurones: beyond magno- and parvocellular cell types? J. Neuroendocrinol. 30, e12549 (2018).
doi: 10.1111/jne.12549
Johnson, Z. V. et al. Central oxytocin receptors mediate mating-induced partner preferences and enhance correlated activation across forebrain nuclei in male prairie voles. Horm. Behav. 79, 8–17 (2016).
pubmed: 26643557 doi: 10.1016/j.yhbeh.2015.11.011 pmcid: 26643557
Hung, L. W. et al. Gating of social reward by oxytocin in the ventral tegmental area. Science 357, 1406–1411 (2017).
pubmed: 28963257 pmcid: 6214365 doi: 10.1126/science.aan4994
Okabe, S., Yoshida, M., Takayanagi, Y. & Onaka, T. Activation of hypothalamic oxytocin neurons following tactile stimuli in rats. Neurosci. Lett. 600, 22–27 (2015).
pubmed: 26033183 doi: 10.1016/j.neulet.2015.05.055 pmcid: 26033183
Bru, T., Salinas, S. & Kremer, E. J. An update on canine adenovirus type 2 and its vectors. Viruses 2, 2134–2153 (2010).
pubmed: 21994722 pmcid: 3185752 doi: 10.3390/v2092134
Gunaydin, L. A. et al. Natural neural projection dynamics underlying social behavior. Cell 157, 1535–1551 (2014).
pubmed: 24949967 pmcid: 4123133 doi: 10.1016/j.cell.2014.05.017
VanRyzin, J. W. et al. Microglial phagocytosis of newborn cells is induced by endocannabinoids and sculpts sex differences in juvenile rat social play. Neuron 102, 435–449.e6 (2019).
pubmed: 30827729 doi: 10.1016/j.neuron.2019.02.006 pmcid: 30827729
Erny, D. et al. Host microbiota constantly control maturation and function of microglia in the CNS. Nat. Neurosci. 18, 965–977 (2015).
pubmed: 26030851 pmcid: 5528863 doi: 10.1038/nn.4030
Wickersham, I. R. et al. Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons. Neuron 53, 639–647 (2007).
pubmed: 17329205 pmcid: 2629495 doi: 10.1016/j.neuron.2007.01.033
Manning, M., Stoev, S., Cheng, L. L., Ching Wo, N. & Chan, W. Y. Design of oxytocin antagonists, which are more selective than atosiban. J. Pept. Sci. 7, 449–465 (2001).
pubmed: 11587184 doi: 10.1002/psc.339 pmcid: 11587184
Grund, T. et al. Neuropeptide S activates paraventricular oxytocin neurons to induce anxiolysis. J. Neurosci. 37, 12214–12225 (2017).
pubmed: 29118105 pmcid: 6596824 doi: 10.1523/JNEUROSCI.2161-17.2017
Rhodes, C. H., Morriell, J. I. & Pfaff, D. W. Immunohistochemical analysis of magnocellular elements in rat hypothalamus: Distribution and numbers of cells containing neurophysin, oxytocin, and vasopressin. J. Comp. Neurol. 198, 45–64 (1981).
pubmed: 7014660 doi: 10.1002/cne.901980106 pmcid: 7014660
Uvnäs-Moberg, K., Handlin, L. & Petersson, M. Self-soothing behaviors with particular reference to oxytocin release induced by non-noxious sensory stimulation. Front. Psychol. 5, 1529 (2015).
pubmed: 25628581 pmcid: 4290532 doi: 10.3389/fpsyg.2014.01529
Walker, S. C., Trotter, P. D., Swaney, W. T., Marshall, A. & Mcglone, F. P. C-tactile afferents: Cutaneous mediators of oxytocin release during affiliative tactile interactions? Neuropeptides 64, 27–38 (2017).
pubmed: 28162847 doi: 10.1016/j.npep.2017.01.001 pmcid: 28162847
Brown, C. H., Bains, J. S., Ludwig, M. & Stern, J. E. Physiological regulation of magnocellular neurosecretory cell activity: integration of intrinsic, local and afferent mechanisms. J. Neuroendocrinol. 25, 678–710 (2013).
pubmed: 23701531 doi: 10.1111/jne.12051 pmcid: 23701531
Hoffman, G. E. & Lyo, D. Anatomical markers of activity in neuroendocrine systems: are we all ‘Fos-ed out’? J. Neuroendocrinol. 14, 259–268 (2002).
pubmed: 11963822 doi: 10.1046/j.1365-2826.2002.00775.x pmcid: 11963822
Hoffman, G. E., Smith, M. S. & Verbalis, J. G. c-Fos and related immediate early gene products as markers of activity in neuroendocrine systems. Front. Neuroendocrinol. 14, 173–213 (1993).
pubmed: 8349003 doi: 10.1006/frne.1993.1006 pmcid: 8349003
Hasan, M. T. et al. A fear memory engram and its plasticity in the hypothalamic oxytocin system. Neuron 103, 133–146.e8 (2019).
pubmed: 31104950 doi: 10.1016/j.neuron.2019.04.029 pmcid: 31104950
Stern, J. E. Electrophysiological and morphological properties of pre-autonomic neurones in the rat hypothalamic paraventricular nucleus. J. Physiol. 537, 161–177 (2001).
pubmed: 11711570 pmcid: 2278924 doi: 10.1111/j.1469-7793.2001.0161k.x
Bosch, O. J. Brain oxytocin correlates with maternal aggression: link to anxiety. J. Neurosci. 25, 6807–6815 (2005).
pubmed: 16033890 pmcid: 6725361 doi: 10.1523/JNEUROSCI.1342-05.2005
Fenelon, V. S., Poulain, D. A. & Theodosis, D. T. Fos synthesis and neuronal activation: analysis of Fos immunoreactivity in identified magnocellular neurons during lactation. Ann. N.Y. Acad. Sci. 689, 508–511 (1993).
pubmed: 8373036 doi: 10.1111/j.1749-6632.1993.tb55580.x pmcid: 8373036
Neumann, I., Douglas, A. J., Pittman, Q. J., Russell, J. A. & Landgraf, R. Oxytocin released within the supraoptic nucleus of the rat brain by positive feedback action is involved in parturition‐related events. J. Neuroendocrinol. 8, 227–233 (1996).
pubmed: 8730656 doi: 10.1046/j.1365-2826.1996.04557.x pmcid: 8730656
Augustine, R. A. et al. Prolactin regulation of oxytocin neurone activity in pregnancy and lactation. J. Physiol. 595, 3591–3605 (2017).
pubmed: 28211122 pmcid: 5451702 doi: 10.1113/JP273712
Kennett, J. E. & McKee, D. T. Oxytocin: an emerging regulator of prolactin secretion in the female rat. J. Neuroendocrinol. 24, 403–412 (2012).
pubmed: 22129099 pmcid: 3288386 doi: 10.1111/j.1365-2826.2011.02263.x
Dölen, G., Darvishzadeh, A., Huang, K. W. & Malenka, R. C. Social reward requires coordinated activity of nucleus accumbens oxytocin and serotonin. Nature 501, 179–184 (2013).
pubmed: 24025838 pmcid: 4091761 doi: 10.1038/nature12518
McGlone, F., Wessberg, J. & Olausson, H. Discriminative and affective touch: sensing and feeling. Neuron 82, 737–755 (2014).
pubmed: 24853935 doi: 10.1016/j.neuron.2014.05.001 pmcid: 24853935
Leng, G. & Ludwig, M. Reply to: Improving research standards to restore trust in intranasal oxytocin. Biol. Psychiatry 79, e55–e56 (2016).
pubmed: 26435221 doi: 10.1016/j.biopsych.2015.08.030 pmcid: 26435221
Meyer-Lindenberg, A., Domes, G., Kirsch, P. & Heinrichs, M. Oxytocin and vasopressin in the human brain: social neuropeptides for translational medicine. Nat. Rev. Neurosci. 12, 524–538 (2011).
pubmed: 21852800 doi: 10.1038/nrn3044 pmcid: 21852800
Grinevich, V. & Neumann, I. D. How puzzle stones from animal studies translate into psychiatry. Mol. Psychiatry https://doi.org/10.1038/s41380-020-0802-9 (2020).
Menon, R. et al. Oxytocin signaling in the lateral septum prevents social fear during lactation. Curr. Biol. 28, 1066–1078.e6 (2018).
pubmed: 29551417 doi: 10.1016/j.cub.2018.02.044 pmcid: 29551417
Grinevich, V. et al. Somatic transgenesis. Viral Vectors 3, 243–274 (2016).
Paxinos, G. & Watson, C. The Rat Brain in Stereotaxic Coordinates, 7th edn (Elsevier Acad. Press, 2014).
Tasker, J. G. & Dudek, F. E. Electrophysiological properties of neurones in the region of the paraventricular nucleus in slices of rat hypothalamus. J. Physiol. 434, 271–293 (1991).
pubmed: 2023120 pmcid: 1181417 doi: 10.1113/jphysiol.1991.sp018469
Chu, C.-P. et al. Effects of stresscopin on rat hypothalamic paraventricular nucleus neurons in vitro. PLoS ONE 8, e53863 (2013).
pubmed: 23349753 pmcid: 3548845 doi: 10.1371/journal.pone.0053863
Luther, J. A. & Tasker, J. G. Voltage-gated currents distinguish parvocellular from magnocellular neurones in the rat hypothalamic paraventricular nucleus. J. Physiol. 523, 193–209 (2000).
pubmed: 10673555 pmcid: 2269788 doi: 10.1111/j.1469-7793.2000.t01-1-00193.x
Luther, J. A. et al. Neurosecretory and non-neurosecretory parvocellular neurones of the hypothalamic paraventricular nucleus express distinct electrophysiological properties. J. Neuroendocrinol. 14, 929–932 (2002).
pubmed: 12472873 doi: 10.1046/j.1365-2826.2002.00867.x pmcid: 12472873
Yuill, E. A., Hoyda, T. D., Ferri, C. C., Zhou, Q.-Y. & Ferguson, A. V. Prokineticin 2 depolarizes paraventricular nucleus magnocellular and parvocellular neurons. Eur. J. Neurosci. 25, 425–434 (2007).
pubmed: 17284183 pmcid: 2667317 doi: 10.1111/j.1460-9568.2006.05293.x
Tang, Y., Benusiglio, D., Grinevich, V. & Lin, L. Distinct types of feeding related neurons in mouse hypothalamus. Front. Behav. Neurosci. 10, 91 (2016).
pubmed: 27242460 pmcid: 4870269
Maícas Royo, J., Brown, C. H., Leng, G. & MacGregor, D. J. Oxytocin neurones: intrinsic mechanisms governing the regularity of spiking activity. J. Neuroendocrinol. 28, 28 (2016).
Grund, T. et al. Chemogenetic activation of oxytocin neurons: temporal dynamics, hormonal release, and behavioral consequences. Psychoneuroendocrinology 106, 77–84 (2019).
pubmed: 30954921 doi: 10.1016/j.psyneuen.2019.03.019 pmcid: 30954921
de Jong, T. R. et al. Salivary oxytocin concentrations in response to running, sexual self-stimulation, breastfeeding and the TSST: the Regensburg Oxytocin Challenge (ROC) study. Psychoneuroendocrinology 62, 381–388 (2015).
pubmed: 26385109 doi: 10.1016/j.psyneuen.2015.08.027 pmcid: 26385109
Landgraf, R., Neumann, I., Holsboer, F. & Pittman, Q. J. Interleukin-1β stimulates both central and peripheral release of vasopressin and oxytocin in the rat. Eur. J. Neurosci. 7, 592–598 (1995).
pubmed: 7620610 doi: 10.1111/j.1460-9568.1995.tb00663.x pmcid: 7620610
Neumann, I. D., Maloumby, R., Beiderbeck, D. I., Lukas, M. & Landgraf, R. Increased brain and plasma oxytocin after nasal and peripheral administration in rats and mice. Psychoneuroendocrinology 38, 1985–1993 (2013).
pubmed: 23579082 doi: 10.1016/j.psyneuen.2013.03.003 pmcid: 23579082
Ishiyama, S. & Brecht, M. Neural correlates of ticklishness in the rat somatosensory cortex. Science 354, 757–760 (2016).
pubmed: 27846607 doi: 10.1126/science.aah5114 pmcid: 27846607
Althammer, F., Ferreira-Neto, H. C., Rubaharan, M., Roy, K. R. & Stern, J. E. Three-dimensional morphometric analysis reveals time-dependent structural changes in microglia and astrocytes in the central amygdala and hypothalamic paraventricular nucleus of heart failure rats. Res. Sq. https://doi.org/10.21203/rs.3.rs-22630/v1 (2020).
Kim, E. J., Jacobs, M. W., Ito-Cole, T. & Callaway, E. M. Improved monosynaptic neural circuit tracing using engineered rabies virus glycoproteins. Cell Rep. 15, 692–699 (2016).
pubmed: 27149846 pmcid: 5063660 doi: 10.1016/j.celrep.2016.03.067
Zhang, B. et al. Reconstruction of the hypothalamo-neurohypophysial system and functional dissection of magnocellular oxytocin neurons in the brain. Preprint at bioRxiv https://doi.org/10.1101/2020.03.26.007070 (2020).

Auteurs

Yan Tang (Y)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
Centre de Neurosciences Psychiatriques, Centre Hospitalier Universitaire Vaudois (CHUV), Prilly (Lausanne), Switzerland.

Diego Benusiglio (D)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Arthur Lefevre (A)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
Centre National de la Recherche Scientifique, Institute of Cellular and Integrative Neurosciences, University of Strasbourg, Strasbourg, France.

Louis Hilfiger (L)

Centre National de la Recherche Scientifique, Institute of Cellular and Integrative Neurosciences, University of Strasbourg, Strasbourg, France.

Ferdinand Althammer (F)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.
Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA, USA.

Anna Bludau (A)

Department of Neurobiology and Animal Physiology, University of Regensburg, Regensburg, Germany.

Daisuke Hagiwara (D)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Angel Baudon (A)

Centre National de la Recherche Scientifique, Institute of Cellular and Integrative Neurosciences, University of Strasbourg, Strasbourg, France.

Pascal Darbon (P)

Centre National de la Recherche Scientifique, Institute of Cellular and Integrative Neurosciences, University of Strasbourg, Strasbourg, France.

Jonas Schimmer (J)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Matthew K Kirchner (MK)

Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA, USA.

Ranjan K Roy (RK)

Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA, USA.

Shiyi Wang (S)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Marina Eliava (M)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany.

Shlomo Wagner (S)

Sagol Department of Neurobiology, University of Haifa, Mount Carmel, Haifa, Israel.

Martina Oberhuber (M)

Max von Pettenkofer-Institute Virology, Faculty of Medicine and Gene Center, Ludwig Maximilian University, Munich, Germany.

Karl K Conzelmann (KK)

Max von Pettenkofer-Institute Virology, Faculty of Medicine and Gene Center, Ludwig Maximilian University, Munich, Germany.

Martin Schwarz (M)

Institute for Experimental Epileptology and Cognition Research, University of Bonn Medical Center, Bonn, Germany.

Javier E Stern (JE)

Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA, USA.

Gareth Leng (G)

Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK.

Inga D Neumann (ID)

Department of Neurobiology and Animal Physiology, University of Regensburg, Regensburg, Germany.

Alexandre Charlet (A)

Centre National de la Recherche Scientifique, Institute of Cellular and Integrative Neurosciences, University of Strasbourg, Strasbourg, France. acharlet@unistra.fr.

Valery Grinevich (V)

Department of Neuropeptide Research in Psychiatry, Central Institute of Mental Health, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany. valery.grinevich@zi-mannheim.de.
Center for Neuroinflammation and Cardiometabolic Diseases, Georgia State University, Atlanta, GA, USA. valery.grinevich@zi-mannheim.de.

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