A simple and reliable analytical method for simultaneous quantification of first line antitubercular drugs in human plasma by LCMS/MS.
Journal
Analytical methods : advancing methods and applications
ISSN: 1759-9679
Titre abrégé: Anal Methods
Pays: England
ID NLM: 101519733
Informations de publication
Date de publication:
21 08 2020
21 08 2020
Historique:
pubmed:
29
7
2020
medline:
15
5
2021
entrez:
29
7
2020
Statut:
ppublish
Résumé
The present study describes the optimization of a simple and reliable method for the determination of four first line antitubercular drugs in human plasma. The studied analytes were isoniazid (H), rifampicin (R), pyrazinamide (Z) and ethambutol (E) in fixed dose combination recommended to patients under the Revised National Tuberculosis Control Programme (RNTCP, India). The analytes were extracted from the human plasma (150 μL) using the single step liquid-liquid extraction approach and were analyzed by liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS). The method was fully validated, according to USFDA guidelines. A linear range of 0.05-10 μg mL
Substances chimiques
Antitubercular Agents
0
Pharmaceutical Preparations
0
Ethambutol
8G167061QZ
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM