Anti-biofilm efficacy of a medieval treatment for bacterial infection requires the combination of multiple ingredients.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 07 2020
28 07 2020
Historique:
received:
11
05
2020
accepted:
16
06
2020
entrez:
30
7
2020
pubmed:
30
7
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Novel antimicrobials are urgently needed to combat drug-resistant bacteria and to overcome the inherent difficulties in treating biofilm-associated infections. Studying plants and other natural materials used in historical infection remedies may enable further discoveries to help fill the antibiotic discovery gap. We previously reconstructed a 1,000-year-old remedy containing onion, garlic, wine, and bile salts, known as 'Bald's eyesalve', and showed it had promising antibacterial activity. In this current paper, we have found this bactericidal activity extends to a range of Gram-negative and Gram-positive wound pathogens in planktonic culture and, crucially, that this activity is maintained against Acinetobacter baumannii, Stenotrophomonas maltophilia, Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus pyogenes in a soft-tissue wound biofilm model. While the presence of garlic in the mixture can explain the activity against planktonic cultures, garlic has no activity against biofilms. We have found the potent anti-biofilm activity of Bald's eyesalve cannot be attributed to a single ingredient and requires the combination of all ingredients to achieve full activity. Our work highlights the need to explore not only single compounds but also mixtures of natural products for treating biofilm infections and underlines the importance of working with biofilm models when exploring natural products for the anti-biofilm pipeline.
Identifiants
pubmed: 32724094
doi: 10.1038/s41598-020-69273-8
pii: 10.1038/s41598-020-69273-8
pmc: PMC7387442
doi:
Substances chimiques
Anti-Bacterial Agents
0
Plant Extracts
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12687Subventions
Organisme : Medical Research Council
ID : MR/N014294/1
Pays : United Kingdom
Organisme : Diabetes UK
ID : 17/0005690
Pays : United Kingdom
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