Evaluation of BP180-NC16A ELISA in exclusive oral pemphigoid diagnosis. A comparative study.
ELISA
pemphigoid
vesiculobullous disease
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
10
06
2020
revised:
04
07
2020
accepted:
19
07
2020
pubmed:
30
7
2020
medline:
3
3
2021
entrez:
30
7
2020
Statut:
ppublish
Résumé
Aims of this study were to test the efficacy of anti-BP180-NC160 ELISA in the diagnosis of oral pemphigoid compared to the gold standard, represented by direct immunofluorescence and pathological examination, to correlate the antibody titers with the severity of the disease and the demographical data. Patients with a suspect of oral pemphigoid were enrolled and underwent biopsy and sera collection both, in order to perform histopathological examination, direct immunofluorescence and ELISA. The test outcomes were compared, and ELISA sensitivity, specificity, accuracy, and negative and positive predictive values were calculated. ELISA showed good specificity (83.3%), while sensitivity was only 50%. A moderate correlation between antibody titers and disease severity was recorded. Mucomembranous Pemphigoid is an autoimmune autoantibody-mediated blistering disease, often affecting exclusively the oral mucosa. Currently, the biopsy is required to diagnose this disease, but serological tests are also commonly employed during clinical practice as adjunctive tools. BP180-NC160 ELISA should be considered an ancillary diagnostic test in course of oral pemphigoid; direct immunofluorescence + histologic examination remains the diagnostic gold standard.
Substances chimiques
Autoantibodies
0
Autoantigens
0
Non-Fibrillar Collagens
0
Types de publication
Journal Article
Langues
eng
Pagination
525-531Informations de copyright
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.
Références
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