RADAR: annotation and prioritization of variants in the post-transcriptional regulome of RNA-binding proteins.


Journal

Genome biology
ISSN: 1474-760X
Titre abrégé: Genome Biol
Pays: England
ID NLM: 100960660

Informations de publication

Date de publication:
30 07 2020
Historique:
received: 03 01 2018
accepted: 28 02 2020
entrez: 31 7 2020
pubmed: 31 7 2020
medline: 7 7 2021
Statut: epublish

Résumé

RNA-binding proteins (RBPs) play key roles in post-transcriptional regulation and disease. Their binding sites cover more of the genome than coding exons; nevertheless, most noncoding variant prioritization methods only focus on transcriptional regulation. Here, we integrate the portfolio of ENCODE-RBP experiments to develop RADAR, a variant-scoring framework. RADAR uses conservation, RNA structure, network centrality, and motifs to provide an overall impact score. Then, it further incorporates tissue-specific inputs to highlight disease-specific variants. Our results demonstrate RADAR can successfully pinpoint variants, both somatic and germline, associated with RBP-function dysregulation, which cannot be found by most current prioritization methods, for example, variants affecting splicing.

Identifiants

pubmed: 32727537
doi: 10.1186/s13059-020-01979-4
pii: 10.1186/s13059-020-01979-4
pmc: PMC7391703
doi:

Substances chimiques

RNA-Binding Proteins 0

Types de publication

Evaluation Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

151

Subventions

Organisme : NIMH NIH HHS
ID : U01 MH116492
Pays : United States

Références

Nature. 2013 Jul 11;499(7457):214-218
pubmed: 23770567
EMBO J. 2012 May 30;31(11):2566-78
pubmed: 22522706
Bioinformatics. 2018 Mar 15;34(6):1031-1033
pubmed: 29121169
Nat Methods. 2014 Mar;11(3):294-6
pubmed: 24487584
Genome Biol. 2014;15(12):550
pubmed: 25516281
Genet Epidemiol. 2011 May;35(4):261-8
pubmed: 21328616
Nucleic Acids Res. 2014 Jan;42(Database issue):D92-7
pubmed: 24297251
Nat Genet. 2013 Jun;45(6):580-5
pubmed: 23715323
Genome Biol. 2014;15(10):480
pubmed: 25273974
Nat Genet. 2013 Oct;45(10):1113-20
pubmed: 24071849
Nat Rev Genet. 2009 Oct;10(10):704-14
pubmed: 19763153
Nat Rev Mol Cell Biol. 2002 Mar;3(3):195-205
pubmed: 11994740
Mol Cell. 2014 Jun 5;54(5):887-900
pubmed: 24837674
Nature. 2015 Feb 19;518(7539):314-6
pubmed: 25693562
Nature. 2012 Nov 1;491(7422):56-65
pubmed: 23128226
Genome Biol. 2006;7 Suppl 1:S4.1-9
pubmed: 16925838
Nat Methods. 2016 Jun;13(6):508-14
pubmed: 27018577
Oncogene. 2010 Feb 18;29(7):1041-9
pubmed: 19915612
Nucleic Acids Res. 2017 Jan 4;45(D1):D104-D114
pubmed: 28053162
EMBO Rep. 2014 Aug;15(8):903-10
pubmed: 24916387
Nat Rev Genet. 2016 Feb;17(2):93-108
pubmed: 26781813
PLoS Comput Biol. 2010 Dec 02;6(12):e1001025
pubmed: 21152010
Nat Rev Genet. 2014 Jul;15(7):469-79
pubmed: 24821474
Adv Exp Med Biol. 2014;825:97-127
pubmed: 25201104
Nucleic Acids Res. 2012 Jan;40(Database issue):D180-6
pubmed: 22086949
Nat Med. 2004 Aug;10(8):789-99
pubmed: 15286780
Bioinformatics. 2011 Jun 15;27(12):1653-9
pubmed: 21543442
Nat Rev Genet. 2014 Dec;15(12):829-45
pubmed: 25365966
Nucleic Acids Res. 2015 Jan;43(Database issue):D160-7
pubmed: 25416797
Nature. 2017 Oct 11;550(7675):204-213
pubmed: 29022597
PLoS Comput Biol. 2013;9(3):e1002886
pubmed: 23505346
Nature. 2012 Sep 6;489(7414):57-74
pubmed: 22955616
Genome Res. 2006 Jul;16(7):912-21
pubmed: 16769980
Science. 2013 Oct 04;342(6154):1235587
pubmed: 24092746
Proc Natl Acad Sci U S A. 2015 Jun 23;112(25):7731-6
pubmed: 26056275
Nature. 2013 Aug 29;500(7464):536-7
pubmed: 23945584
Nat Rev Genet. 2014 Oct;15(10):689-701
pubmed: 25112293
Genome Res. 2005 Jul;15(7):901-13
pubmed: 15965027
PLoS Comput Biol. 2006 Apr;2(4):e33
pubmed: 16628248
Curr Opin Genet Dev. 2014 Feb;24:52-60
pubmed: 24657537
Genome Res. 2012 Sep;22(9):1760-74
pubmed: 22955987
Nat Genet. 2014 Mar;46(3):310-5
pubmed: 24487276
Nature. 2015 Oct 1;526(7571):68-74
pubmed: 26432245
BMC Genomics. 2017 Jan 3;18(1):5
pubmed: 28049435
Nature. 2013 Aug 22;500(7463):415-21
pubmed: 23945592
Nucleic Acids Res. 2015 Sep 30;43(17):8123-34
pubmed: 26304545
Biochim Biophys Acta. 2014 Nov;1839(11):1097-109
pubmed: 25159663
Curr Protoc Hum Genet. 2016 Oct 11;91:10.11.1-10.11.37
pubmed: 27727438
FEBS Lett. 2008 Jun 18;582(14):1977-86
pubmed: 18342629
Nat Rev Cancer. 2011 Aug 05;11(9):644-56
pubmed: 21822212

Auteurs

Jing Zhang (J)

Department of Computer Science, University of California, Irvine, CA, 92697, USA.

Jason Liu (J)

Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT, 06520, USA.
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06520, USA.

Donghoon Lee (D)

Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT, 06520, USA.

Jo-Jo Feng (JJ)

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06520, USA.

Lucas Lochovsky (L)

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06520, USA.

Shaoke Lou (S)

Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT, 06520, USA.
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06520, USA.

Michael Rutenberg-Schoenberg (M)

Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT, 06520, USA.
Chemical Biology Institute, Yale University, West Haven, CT, 06516, USA.

Mark Gerstein (M)

Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT, 06520, USA. mark@gersteinlab.org.
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06520, USA. mark@gersteinlab.org.
Department of Computer Science, Yale University, New Haven, CT, 06520, USA. mark@gersteinlab.org.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH