Lipoteichoic Acid as a Potential Noninvasive Biomarker of Biofilm in Dialysis Access.
Aged
Arteriovenous Shunt, Surgical
/ adverse effects
Biofilms
Biomarkers
/ blood
Central Venous Catheters
/ adverse effects
Female
Gram-Positive Bacterial Infections
/ blood
Humans
Lipopolysaccharides
/ blood
Male
Middle Aged
Pilot Projects
Prosthesis-Related Infections
/ blood
Renal Dialysis
/ adverse effects
Teichoic Acids
/ blood
Treatment Outcome
Journal
ASAIO journal (American Society for Artificial Internal Organs : 1992)
ISSN: 1538-943X
Titre abrégé: ASAIO J
Pays: United States
ID NLM: 9204109
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
entrez:
3
8
2020
pubmed:
3
8
2020
medline:
20
1
2021
Statut:
ppublish
Résumé
Tunneled central venous catheters (TCVCs) are colonized by Gram-positive organisms and form biofilm. Lipoteichoic acid (LTA) is a Gram-positive cell wall component that can be measured in serum. The purpose of this pilot study was to characterize LTA concentrations in hemodialysis (HD) patients with TCVCs compared to other access types and to evaluate biofilm morphology and microbiology in TCVCs removed by clinical decision. The study enrolled patients with TCVCs (18), grafts (19), and fistulas (18). Blood samples were collected before HD, at 30 minutes, 2 hours, and end of HD. Catheters removed by clinical decision were evaluated by scanning electron microscopy (SEM) for biofilm morphology, and portions of the catheter were cultured. LTA was detectable in all samples and concentrations increased significantly in all access types during HD (p < 0.05 for all comparisons). Patients with TCVCs that had a >30% increase in LTA concentration from baseline also had the greatest rate of increase (slope) compared to grafts and fistulas (p = 0.03 and p = 0.04, respectively). Catheters removed by clinical decision (n = 7) and examined by SEM had deposition of fibrin. Cultures revealed polymicrobial colonization. TCVCs had the highest rate of increase of LTA during HD. Further studies to determine the source of LTA in patients with AVG and AVF are warranted.
Identifiants
pubmed: 32740359
doi: 10.1097/MAT.0000000000001091
pii: 00002480-202008000-00021
doi:
Substances chimiques
Biomarkers
0
Lipopolysaccharides
0
Teichoic Acids
0
lipoteichoic acid
56411-57-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
960-965Références
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