A human ESC-based screen identifies a role for the translated lncRNA
CRISPR-Cas Systems
Cell Differentiation
/ physiology
Cells, Cultured
Gene Deletion
Gene Expression Regulation, Developmental
Gene Knockout Techniques
HEK293 Cells
Human Embryonic Stem Cells
Humans
Islets of Langerhans
/ cytology
Male
Protein Biosynthesis
RNA, Long Noncoding
/ genetics
Transcription Factors
/ metabolism
ORF detection
computational biology
developmental biology
endocrine development
endoderm
human
lncRNA
microproteins
pancreas
systems biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
03 08 2020
03 08 2020
Historique:
received:
07
05
2020
accepted:
01
08
2020
pubmed:
4
8
2020
medline:
20
2
2021
entrez:
4
8
2020
Statut:
epublish
Résumé
Long noncoding RNAs (lncRNAs) are a heterogenous group of RNAs, which can encode small proteins. The extent to which developmentally regulated lncRNAs are translated and whether the produced microproteins are relevant for human development is unknown. Using a human embryonic stem cell (hESC)-based pancreatic differentiation system, we show that many lncRNAs in direct vicinity of lineage-determining transcription factors (TFs) are dynamically regulated, predominantly cytosolic, and highly translated. We genetically ablated ten such lncRNAs, most of them translated, and found that nine are dispensable for pancreatic endocrine cell development. However, deletion of
Identifiants
pubmed: 32744504
doi: 10.7554/eLife.58659
pii: 58659
pmc: PMC7423336
doi:
pii:
Substances chimiques
RNA, Long Noncoding
0
Transcription Factors
0
Banques de données
GEO
['GSE144682', 'GSE30611', 'GSE33480', 'GSE93448', 'GSE34995', 'GSE90322', 'GSE93435', 'GSE43770', 'GSE93450', 'GSE93451', 'GSE90176']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK068471
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK063491
Pays : United States
Organisme : Larry L. Hillblom Foundation
ID : 2015-D-021-FEL
Pays : International
Organisme : NIDDK NIH HHS
ID : R01 DK078803
Pays : United States
Organisme : Horizon 2020 Framework Programme
ID : AdG788970
Pays : International
Organisme : European Molecular Biology Organization
ID : ALTF 186-2015
Pays : International
Informations de copyright
© 2020, Gaertner et al.
Déclaration de conflit d'intérêts
BG, Sv, VS, JS, FW, SB, SN, RW, IM, NH, MS No competing interests declared
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