Bone Morphogenetic Protein-2-Activated 3D-Printed Polylactic Acid Scaffolds to Promote Bone Regrowth and Repair.
3D printing
bone morphogenetic protein-2
bone regeneration
bone scaffold
polylactic acid
stem cells
Journal
Macromolecular bioscience
ISSN: 1616-5195
Titre abrégé: Macromol Biosci
Pays: Germany
ID NLM: 101135941
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
05
05
2020
revised:
28
06
2020
pubmed:
5
8
2020
medline:
2
9
2021
entrez:
5
8
2020
Statut:
ppublish
Résumé
Uneven distribution of pores, lack of connection between holes, low reproducibility, insufficient mechanical strength, and incomplete volatility of organic solvents are some problems associated with traditional tissue engineering methods for bone defect repair. These characteristics reduce the quality and stability of products. This study uses 3D printing (3DP) to fabricate a biocompatible poly(lactic) acid-based scaffold for repairing bone tissue. Hence, three different types of scaffolds are assessed: a freeze-dried polylactic acid (PLA) scaffold constructed using the traditional freeze-extraction method; a 3D-PLA scaffold produced through the 3DP technique; and a 3D-PLA-bone morphogenetic protein-2 (BMP-2) scaffold that is prepared using 3DP technology, with the addition of BMP-2. To enhance biological activity, polydopamine (pDA) is used to graft BMP-2 on the surface of the 3D-PLA-BMP-2 scaffold. Then, the scaffolds are implanted into the bilateral femoral condyles of rabbits, and their ability to repair the bone tissue defects is tested. The results of the experiments reveal that the 3DP scaffolds are more biocompatible than the ones produced through the traditional manufacturing methods because they enhance cell adhesion and differentiation after pDA modification and BMP-2 fixation. In the future, the 3DP products may be applied for the repair of larger bone defects in the clinical setting.
Identifiants
pubmed: 32749079
doi: 10.1002/mabi.202000161
doi:
Substances chimiques
Bone Morphogenetic Protein 2
0
Polyesters
0
poly(lactide)
459TN2L5F5
Alkaline Phosphatase
EC 3.1.3.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000161Informations de copyright
© 2020 Wiley-VCH GmbH.
Références
M. Mastrogiacomo, A. Muraglia, V. Komlev, F. Peyrin, F. Rustichelli, A. Crovace, R. Cancedda, Orthod. Craniofacial Res. 2005, 8, 277.
E. M. Younger, M. W. Chapman, J. Orthop. Trauma 1989, 3, 192.
E. Ahlmann, M. Patzakis, N. Roidis, L. Shepherd, P. Holtom, J. Bone Jt. Surg. 2002, 84, 716.
R. Alluri, X. Song, S. Bougioukli, W. Pannell, V. Vakhshori, O. Sugiyama, A. Tang, S. H. Park, Y. Chen, J. R. Lieberman, J. Biomed. Mater. Res., Part A 2019, 107, 2174.
R. A. Giordano, B. M. Wu, S. W. Borland, L. G. Cima, E. M. Sachs, M. J. Cima, J. Biomater. Sci., Polym. Ed. 1997, 8, 63.
S. S. Athukoralalage, R. Balu, N. K. Dutta, N. Roy Choudhury, Polymers 2019, 11, 898.
J. Garcia, Z. Yang, R. Mongrain, R. L. Leask, K. Lachapelle, BMJ Simul. Technol. Enhanced Learn. 2018, 4, 27.
J. W. Lee, K. S. Kang, S. H. Lee, J. Y. Kim, B. K. Lee, D. W. Cho, Biomaterials 2011, 32, 744.
S. V. Murphy, A. Atala, Nat. Biotechnol. 2014, 32, 773.
R. Fairag, D. H. Rosenzweig, J. L. Ramirez-Garcialuna, M. H. Weber, L. Haglund, ACS Appl. Mater. Interfaces 2019, 11, 15306.
A. R. Studart, Chem. Soc. Rev. 2016, 45, 359.
M. H. Ho, P. Y. Kuo, H. J. Hsieh, T. Y. Hsien, L. T. Hou, J. Y. Lai, D. M. Wang, Biomaterials 2004, 25, 129.
C. Schugens, V. Maquet, C. Grandfils, R. Jerome, P. Teyssie, J. Biomed. Mater. Res. 1996, 30, 449.
F. J. Hua, G. E. Kim, J. D. Lee, Y. K. Son, D. S. Lee, J. Biomed. Mater. Res. 2002, 63, 161.
L. Budyanto, Y. Q. Goh, C. P. Ooi, J. Mater. Sci.: Mater. Med. 2009, 20, 105.
S. Cardea, L. Baldino, P. Pisanti, E. Reverchon, J. Mater. Sci.: Mater. Med. 2014, 25, 355.
G. Li, S. Qin, D. Zhang, X. Liu, Nanotechnology 2019, 30, 295101.
K. A. Athanasiou, G. G. Niederauer, C. M. Agrawal, Biomaterials 1996, 17, 93.
U. Ritz, R. Gerke, H. Gotz, S. Stein, P. M. Rommens, Int. J. Mol. Sci. 2017, 18, 2569.
J. Hu, X. Liu, P. X. Ma, Biomaterials 2008, 29, 3815.
M. S. Taylor, A. U. Daniels, K. P. Andriano, J. Heller, J. Appl. Biomater. 1994, 5, 151.
D. Y. Hwang, S. W. On, S. I. Song, Maxillofacial Plast. Reconstr. Surg. 2016, 38, 22.
M. Cicciu, J. Craniofacial Surg. 2017, 28, 592.
P. Zhou, J. Wu, Y. Xia, Y. Yuan, H. Zhang, S. Xu, K. Lin, Int. J. Nanomed. 2018, 13, 4083.
I. W. Um, S. H. Hwang, Y. K. Kim, M. Y. Kim, S. H. Jun, J. J. Ryu, H. S. Jang, J. Korean Assoc. Oral Maxillofacial Surg. 2016, 42, 90.
Y. Castro-Govea, V. H. Cervantes-Kardasch, G. Borrego-Soto, H. G. Martinez-Rodriguez, M. Espinoza-Juarez, V. Romero-Diaz, I. A. Marino-Martinez, A. Robles-Zamora, E. Alvarez-Lozano, G. R. Padilla-Rivas, R. Ortiz-Lopez, J. Lara-Arias, J. Vazquez-Juarez, A. Rojas-Martinez, J. Craniofacial Surg. 2012, 23, 392.
O. Hanisch, D. N. Tatakis, M. M. Boskovic, M. D. Rohrer, U. M. Wikesjo, Int. J. Oral Maxillofacial Implants 1997, 12, 604.
Y. Ding, X. Wang, J. Craniofacial Surg. 2018, 29, e58.
E. Jang, J. Y. Lee, E. Y. Lee, H. Seok, Materials 2019, 12, 1613.
H. J. Cho, S. K. Perikamana, J. H. Lee, J. Lee, K. M. Lee, C. S. Shin, H. Shin, ACS Appl. Mater. Interfaces 2014, 6, 11225.
S. Jo, S. M. Kang, S. A. Park, W. D. Kim, J. Kwak, H. Lee, Macromol. Biosci. 2013, 13, 1389.
J. Kang, S. Tada, T. Kitajima, T. I. Son, T. Aigaki, Y. Ito, Biomed. Res. Int. 2013, 2013, 265980.
W. B. Tsai, W. T. Chen, H. W. Chien, W. H. Kuo, M. J. Wang, J. Biomater. Appl. 2014, 28, 837.
E. Ko, K. Yang, J. Shin, S. W. Cho, Biomacromolecules 2013, 14, 3202.
K. Yang, J. S. Lee, J. Kim, Y. B. Lee, H. Shin, S. H. Um, J. B. Kim, K. I. Park, H. Lee, S. W. Cho, Biomaterials 2012, 33, 6952.
X. Yu, J. Walsh, M. Wei, RSC Adv. 2014, 4, 7185.
S. J. Weng, D. Y. Yan, L. J. Gu, L. Chen, Z. J. Xie, Z. Y. Wu, J. H. Tang, Z. J. Shen, H. Li, B. L. Bai, V. Boodhun, L. Yang, Chem. Biol. Interact. 2019, 300, 101.
H. E. Gruber, Biotech. Histochem. 1992, 67, 30.
C. H. Cheng, Y. W. Chen, A. K.-X. Lee, C. H. Yao, M. Y. Shie, J. Mater. Sci. 2019, 30, 78.
C. T. Kao, C. C. Lin, Y. W. Chen, C. H. Yeh, H. Y. Fang, M. Y. Shie, Mater. Sci. Eng., C 2015, 56, 165.